Arabidopsis Cyclin-Dependent Kinase Gene CDKG;2 is Involved in Organogenic Responses Induced in Vitro

Arabidopsis Cyclin-Dependent Kinase Gene CDKG;2 is Involved in Organogenic Responses Induced in... Abstract The Arabidopsis CDKG;2 gene encodes a putative cyclin-dependent Ser/Thr protein kinase of unknown biological function. This gene shows structural similarity to animal and human cyclin-dependent (PITSLRE) kinases. This study used the homozygous knockout cdkg;2 mutant based on T-DNA insertional line SALK_090262 to study the effect of mutation of the CDKG;2 gene on explant response and in vitro plant regeneration. For callus induction and proliferation, hypocotyls and cotyledons of 3-day-old seedlings of cdkg;2 and A. thaliana ecotype Col-0 were cultured on solid MS medium supplemented with 2,4-D (2 mg l -1 ). Organogenesis was induced after callus transfer on MS + TDZ (0.5 mg l -1 ). The initiation time of callus and shoot induction differed between the mutant and control cultures. Shoot regeneration after callus transfer on MS + TDZ was delayed in cdkg;2 (31 days versus 7 days in Col- 0). Shoots formed on callus derived from Col-0 hypocotyls but not on cotyledon-derived callus; in cdkg;2, shoots developed on both callus types. Mutant shoots did not form roots, regenerants were dwarfed, and inflorescences had small bud-like flowers with a reduced corolla and generative organs. Abnormalities observed during cdkg;2 organogenesis suggest a role of CDKG;2 as a regulator of adventitious root initiation http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Biologica Cracoviensia s. Botanica de Gruyter

Arabidopsis Cyclin-Dependent Kinase Gene CDKG;2 is Involved in Organogenic Responses Induced in Vitro

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Publisher
de Gruyter
Copyright
Copyright © 2013 by the
ISSN
0001-5296
eISSN
1898-0295
D.O.I.
10.2478/abcsb-2013-0003
Publisher site
See Article on Publisher Site

Abstract

Abstract The Arabidopsis CDKG;2 gene encodes a putative cyclin-dependent Ser/Thr protein kinase of unknown biological function. This gene shows structural similarity to animal and human cyclin-dependent (PITSLRE) kinases. This study used the homozygous knockout cdkg;2 mutant based on T-DNA insertional line SALK_090262 to study the effect of mutation of the CDKG;2 gene on explant response and in vitro plant regeneration. For callus induction and proliferation, hypocotyls and cotyledons of 3-day-old seedlings of cdkg;2 and A. thaliana ecotype Col-0 were cultured on solid MS medium supplemented with 2,4-D (2 mg l -1 ). Organogenesis was induced after callus transfer on MS + TDZ (0.5 mg l -1 ). The initiation time of callus and shoot induction differed between the mutant and control cultures. Shoot regeneration after callus transfer on MS + TDZ was delayed in cdkg;2 (31 days versus 7 days in Col- 0). Shoots formed on callus derived from Col-0 hypocotyls but not on cotyledon-derived callus; in cdkg;2, shoots developed on both callus types. Mutant shoots did not form roots, regenerants were dwarfed, and inflorescences had small bud-like flowers with a reduced corolla and generative organs. Abnormalities observed during cdkg;2 organogenesis suggest a role of CDKG;2 as a regulator of adventitious root initiation

Journal

Acta Biologica Cracoviensia s. Botanicade Gruyter

Published: Jun 1, 2013

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