An Ideal Substrate for the Measurement of Pancreatic Amylase?

An Ideal Substrate for the Measurement of Pancreatic Amylase? Abstract The advanced knowledge on substrate cleavage by human α-amylases promotes the development of chromogenic maltotriosides exclusively cleaved at the aglycone bond. Three essentials are required for this type of binding at the active site of the enzyme: (i) A minimal hydrophobic modification at the ultimate glucose unit to exclude the condensation of reaction products, (ii) a non-ionic substituent in the 2-position of the phenolic chromophore, and (iii) pertinent effectors to accomodate the aglycone at subsite +1. The novel substrate 2-chloro-4-nitrophenyl-α-D-maltotrioside (acG3-CNP) is presented as an example together with measurement conditions which allow a direct, sensitive and specific measurement of pancreatic amylase without stoichiometric calculations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

An Ideal Substrate for the Measurement of Pancreatic Amylase?

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Publisher
de Gruyter
Copyright
Copyright © 2002 by the
ISSN
1434-6621
DOI
10.1515/CCLM.2002.134
pmid
12392304
Publisher site
See Article on Publisher Site

Abstract

Abstract The advanced knowledge on substrate cleavage by human α-amylases promotes the development of chromogenic maltotriosides exclusively cleaved at the aglycone bond. Three essentials are required for this type of binding at the active site of the enzyme: (i) A minimal hydrophobic modification at the ultimate glucose unit to exclude the condensation of reaction products, (ii) a non-ionic substituent in the 2-position of the phenolic chromophore, and (iii) pertinent effectors to accomodate the aglycone at subsite +1. The novel substrate 2-chloro-4-nitrophenyl-α-D-maltotrioside (acG3-CNP) is presented as an example together with measurement conditions which allow a direct, sensitive and specific measurement of pancreatic amylase without stoichiometric calculations.

Journal

Clinical Chemistry and Laboratory Medicine (CCLM)de Gruyter

Published: Aug 26, 2002

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