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A sustainable process for enzymatic saccharification of ionic liquid-pretreated cellulosic materials

A sustainable process for enzymatic saccharification of ionic liquid-pretreated cellulosic materials Abstract A two-step sustainable process for the enzymatic saccharification of lignocellulosic materials (e.g., crystalline cellulose, paper and corn cob) was evaluated. In the first step, pretreatment of crystalline cellulose, paper and lignocellulose by the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride, (Bmim)(Cl), was carried out to obtain an amorphous substrate. This step included the recovery of the IL for further reuse. Then, the amorphous cellulosic materials were hydrolyzed by different industrial enzyme preparations. The pretreatment of cellulose, paper and lignocellulose (corn cob) with (Bmim)(Cl) led to an up to 2.5-fold increase in the yield of the enzymatic hydrolysis, compared to the same substrate without pretreatment; this produced nearly 100% hydrolysis. The combined action of Celluclast 1.5L (cellulase from Trichoderma reesei ) and Novozyme 188 (cellobiase from Aspergillus niger ) was essential in the process of enzymatic saccharification of corn cob. By combining the enzyme complex Ultraflo L (β-endoglucanase from Humicola insolens mixed with arabanase and xylanase enzymes) with Celluclast 1.5L and Novozyme 188, the soluble sugar concentration was increased 1.5-fold, resulting in the best choice for enzymatic saccharification of lignocellulosic materials. The effectiveness of lignocellulose pretreatment with (Bmim)(Cl) for improving enzymatic saccharification yield was also confirmed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Green Processing and Synthesis de Gruyter

A sustainable process for enzymatic saccharification of ionic liquid-pretreated cellulosic materials

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Publisher
de Gruyter
Copyright
Copyright © 2014 by the
ISSN
2191-9542
eISSN
2191-9550
DOI
10.1515/gps-2014-0055
Publisher site
See Article on Publisher Site

Abstract

Abstract A two-step sustainable process for the enzymatic saccharification of lignocellulosic materials (e.g., crystalline cellulose, paper and corn cob) was evaluated. In the first step, pretreatment of crystalline cellulose, paper and lignocellulose by the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride, (Bmim)(Cl), was carried out to obtain an amorphous substrate. This step included the recovery of the IL for further reuse. Then, the amorphous cellulosic materials were hydrolyzed by different industrial enzyme preparations. The pretreatment of cellulose, paper and lignocellulose (corn cob) with (Bmim)(Cl) led to an up to 2.5-fold increase in the yield of the enzymatic hydrolysis, compared to the same substrate without pretreatment; this produced nearly 100% hydrolysis. The combined action of Celluclast 1.5L (cellulase from Trichoderma reesei ) and Novozyme 188 (cellobiase from Aspergillus niger ) was essential in the process of enzymatic saccharification of corn cob. By combining the enzyme complex Ultraflo L (β-endoglucanase from Humicola insolens mixed with arabanase and xylanase enzymes) with Celluclast 1.5L and Novozyme 188, the soluble sugar concentration was increased 1.5-fold, resulting in the best choice for enzymatic saccharification of lignocellulosic materials. The effectiveness of lignocellulose pretreatment with (Bmim)(Cl) for improving enzymatic saccharification yield was also confirmed.

Journal

Green Processing and Synthesisde Gruyter

Published: Oct 1, 2014

References