Introduction Glycosaminoglycans, which are widely distributed in human tissues and fluids (1), are intimately related to proteoglycan metabolism. In plasma, glycosamino. , ./. , i glycans have a wide range of molecular masses, but a large proportion have relative molecular masses of less than 5000 (2). Various laboratories have demon^ , ~" . x strated Al presence of glycosaminoglycans in 1human the plasma or serum (2-5). The results indicate that a great quantitative variability may exist among different methods for determination of blood glycosaminoglycans. _ , j t j. Recently we measured the glycosaminoglycan disaccharides from human blood donors, using two differ, , ' . . . . . c . Materials and Methods Reagents Chondroitinase AC (EC 126.96.36.199)1) of Anhrobacter aurescens 01 88301) and chondroitinase ABC (EC 188.8.131.52) of Proteus vulgar is (lot E88301) were purchased from Seikagaku Kogyo (T(fky0j Japan) a.Mannosidase (EC 184.108.40.206) of Canavalia ensiformis Got 48F-9545) was from Sigma (St. Louis, USA) and PaP*in -galactose ( Di4S), 2-acetamido-2-deoxy-3-O-( -Z)-gluco;4-enepyranosyluronic acid)-6-O-sulpho-Z>-galactose ( Di6S) and 92) from Boehrmger Mannheim (Mannheim, Germany), ent methods for isolation of glycosaminoglycans in plasma and serum. The Chromatographie conditions are identical for both methods of glycosaminoglycan isolation. ) Enzymes: Significant sex
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1993
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera