A High Performance Liquid Chromatography Method for the Determination of Glycosaminoglycans in Human Blood

A High Performance Liquid Chromatography Method for the Determination of Glycosaminoglycans in... Introduction Glycosaminoglycans, which are widely distributed in human tissues and fluids (1), are intimately related to proteoglycan metabolism. In plasma, glycosamino. , ./. , i glycans have a wide range of molecular masses, but a large proportion have relative molecular masses of less than 5000 (2). Various laboratories have demon^ , ~" . x strated Al presence of glycosaminoglycans in 1human the plasma or serum (2-5). The results indicate that a great quantitative variability may exist among different methods for determination of blood glycosaminoglycans. _ , j t j. Recently we measured the glycosaminoglycan disaccharides from human blood donors, using two differ, , ' . . . . . c . Materials and Methods Reagents Chondroitinase AC (EC 4.2.2.5)1) of Anhrobacter aurescens 01 88301) and chondroitinase ABC (EC 4.2.2.4) of Proteus vulgar is (lot E88301) were purchased from Seikagaku Kogyo (T(fky0j Japan) a.Mannosidase (EC 3.2.1.24) of Canavalia ensiformis Got 48F-9545) was from Sigma (St. Louis, USA) and PaP*in -galactose ( Di4S), 2-acetamido-2-deoxy-3-O-( -Z)-gluco;4-enepyranosyluronic acid)-6-O-sulpho-Z>-galactose ( Di6S) and 92) from Boehrmger Mannheim (Mannheim, Germany), ent methods for isolation of glycosaminoglycans in plasma and serum. The Chromatographie conditions are identical for both methods of glycosaminoglycan isolation. ) Enzymes: Significant sex http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

A High Performance Liquid Chromatography Method for the Determination of Glycosaminoglycans in Human Blood

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.8.503
Publisher site
See Article on Publisher Site

Abstract

Introduction Glycosaminoglycans, which are widely distributed in human tissues and fluids (1), are intimately related to proteoglycan metabolism. In plasma, glycosamino. , ./. , i glycans have a wide range of molecular masses, but a large proportion have relative molecular masses of less than 5000 (2). Various laboratories have demon^ , ~" . x strated Al presence of glycosaminoglycans in 1human the plasma or serum (2-5). The results indicate that a great quantitative variability may exist among different methods for determination of blood glycosaminoglycans. _ , j t j. Recently we measured the glycosaminoglycan disaccharides from human blood donors, using two differ, , ' . . . . . c . Materials and Methods Reagents Chondroitinase AC (EC 4.2.2.5)1) of Anhrobacter aurescens 01 88301) and chondroitinase ABC (EC 4.2.2.4) of Proteus vulgar is (lot E88301) were purchased from Seikagaku Kogyo (T(fky0j Japan) a.Mannosidase (EC 3.2.1.24) of Canavalia ensiformis Got 48F-9545) was from Sigma (St. Louis, USA) and PaP*in -galactose ( Di4S), 2-acetamido-2-deoxy-3-O-( -Z)-gluco;4-enepyranosyluronic acid)-6-O-sulpho-Z>-galactose ( Di6S) and 92) from Boehrmger Mannheim (Mannheim, Germany), ent methods for isolation of glycosaminoglycans in plasma and serum. The Chromatographie conditions are identical for both methods of glycosaminoglycan isolation. ) Enzymes: Significant sex

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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