Biol. Chem. Hoppe-Seyler Vol. 374, pp. 217-225, April 1993 44. Mosbacher Kolloquium Glyco- and Cellbiology Biosynthesis, Transport and Function of Glycoconjugates April 22th to 24th, 1993 Abstracts MO1 MO 2 T.A. Rapoport Components and Mechanisms Involved in Protein Translocation across the ER Membrane The process of protein translocation can be divided into two phases: 1. an initiation or targeting process, which generally involves the function of the signal recognition particle (SRP) and of its receptor, and 2. the actual transfer of the polypeptide across the membrane which is only poorly understood. We have used chemical crosslinking to identify membrane proteins which are adjacent to nascent polypeptide chains as they pass through the membrane. A combination of crosslinking and reconstitution methods led to the identification of a multi-spanning membrane protein, the "translocating chain associating membrane (TRAM)" protein'''.TheTRAM protein is in vicinity of polypeptides early during their membrane transfer and it is stimulatory or required for the translocation of different secretory proteins.The main component of a putative protein-conducting channel, however, seems to be Secolp. a membrane protein originally discovered in Saccharomyces cerevisiae by genetic methods. Secolp is in proximity of translocating polypeptides throughout their membrane transfer both in yeast'2·3' and
Biological Chemistry Hoppe-Seyler – de Gruyter
Published: Jan 1, 1993
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