Biol. Chem. Hoppe-Seyler Vol. 374, pp. 143-170, March 1993 103. Konferenz der Gesellschaft für Biologische Chemie Studiengruppe Membranstruktur und -transport Membranforum Frankfurt Frankfurt/Main, April 5th-7th, 1993 Organized by Prof. Dr. Gerhard Burckardt, Zentrum Physiologie und Pathophysiologie, Humboldtallee 23, VV-3400 Göttingen and Prof. Dr. Dr. Hugo Fasold, Institut für Biochemie, Klinikum, Theodor-Stern-Kai 7, VV-6000 Frankfurt/Main 70 Abstracts MP Vitality status of cells in multicellular spheroids M. Wartenberg, H. Acker. Max-Planck-Institut für Systemphysiologie, 4600 Dortmund l, FRG We like to discuss the well-known model of "three distinct concentric annular shells" for cancer spheroids grown in a suspension culture with respect to new data given from optical sectioning with the aid of CLSM of spheroids loaded with fluorescence dyes. Cells of different growth activity may respond in their own characteristic fashion with a characteristic sensitivity to various fluorescent vital/lethal dyes. As a vital dye Fluoresceindiacetate (FDA) was used to detect the rim of proliferating cells and as lethal dye Lucifer Yellow VS as well as Lucifer Yellow CH were tested to mark the necrotic core. The evolution of the spheroids and the development of the necrosis can be described with these fluorescent markers. In the outermost shell cells are observed to
Biological Chemistry Hoppe-Seyler – de Gruyter
Published: Jan 1, 1993
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