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Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection

Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection <jats:sec> <jats:title content-type="abstract-subheading">Purpose</jats:title> <jats:p>The purpose of this study is to fabricate a transducer-based TiO<jats:sub>2</jats:sub> interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for <jats:italic>Escherichia. coli</jats:italic> O157:H7 DNA detection.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Design/methodology/approach</jats:title> <jats:p>Sol-gel was used to synthesize TiO<jats:sub>2</jats:sub> nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Findings</jats:title> <jats:p>TiO<jats:sub>2</jats:sub> nanoparticles IDEs with 16-um gap size is able to detect DNA of <jats:italic>E. coli</jats:italic> in a dry state.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Originality/value</jats:title> <jats:p>This paper describes IDEs for dry-state detection of <jats:italic>E. coli</jats:italic> O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.</jats:p> </jats:sec> http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microelectronics International CrossRef

Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection

Microelectronics International , Volume 34 (2): 99-107 – May 2, 2017

Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection


Abstract

<jats:sec>
<jats:title content-type="abstract-subheading">Purpose</jats:title>
<jats:p>The purpose of this study is to fabricate a transducer-based TiO<jats:sub>2</jats:sub> interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for <jats:italic>Escherichia. coli</jats:italic> O157:H7 DNA detection.</jats:p>
</jats:sec>
<jats:sec>
<jats:title content-type="abstract-subheading">Design/methodology/approach</jats:title>
<jats:p>Sol-gel was used to synthesize TiO<jats:sub>2</jats:sub> nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.</jats:p>
</jats:sec>
<jats:sec>
<jats:title content-type="abstract-subheading">Findings</jats:title>
<jats:p>TiO<jats:sub>2</jats:sub> nanoparticles IDEs with 16-um gap size is able to detect DNA of <jats:italic>E. coli</jats:italic> in a dry state.</jats:p>
</jats:sec>
<jats:sec>
<jats:title content-type="abstract-subheading">Originality/value</jats:title>
<jats:p>This paper describes IDEs for dry-state detection of <jats:italic>E. coli</jats:italic> O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.</jats:p>
</jats:sec>

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Publisher
CrossRef
ISSN
1356-5362
DOI
10.1108/mi-08-2016-0054
Publisher site
See Article on Publisher Site

Abstract

<jats:sec> <jats:title content-type="abstract-subheading">Purpose</jats:title> <jats:p>The purpose of this study is to fabricate a transducer-based TiO<jats:sub>2</jats:sub> interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for <jats:italic>Escherichia. coli</jats:italic> O157:H7 DNA detection.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Design/methodology/approach</jats:title> <jats:p>Sol-gel was used to synthesize TiO<jats:sub>2</jats:sub> nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Findings</jats:title> <jats:p>TiO<jats:sub>2</jats:sub> nanoparticles IDEs with 16-um gap size is able to detect DNA of <jats:italic>E. coli</jats:italic> in a dry state.</jats:p> </jats:sec> <jats:sec> <jats:title content-type="abstract-subheading">Originality/value</jats:title> <jats:p>This paper describes IDEs for dry-state detection of <jats:italic>E. coli</jats:italic> O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.</jats:p> </jats:sec>

Journal

Microelectronics InternationalCrossRef

Published: May 2, 2017

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