Abstract

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<jats:title content-type="abstract-subheading">Purpose</jats:title>
<jats:p>The purpose of this study is to fabricate a transducer-based TiO<jats:sub>2</jats:sub> interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for <jats:italic>Escherichia. coli</jats:italic> O157:H7 DNA detection.</jats:p>
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<jats:title content-type="abstract-subheading">Design/methodology/approach</jats:title>
<jats:p>Sol-gel was used to synthesize TiO<jats:sub>2</jats:sub> nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.</jats:p>
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<jats:title content-type="abstract-subheading">Findings</jats:title>
<jats:p>TiO<jats:sub>2</jats:sub> nanoparticles IDEs with 16-um gap size is able to detect DNA of <jats:italic>E. coli</jats:italic> in a dry state.</jats:p>
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<jats:title content-type="abstract-subheading">Originality/value</jats:title>
<jats:p>This paper describes IDEs for dry-state detection of <jats:italic>E. coli</jats:italic> O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.</jats:p>
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