VARIATION IN SEX RATIO OF PANAGRELLUS REDIVIVUS IN RESPONSE TO NUTRITIONAL AND HEAT STRESS BY EDER L. HANSEN 1) AND WILLIAM S. CRYAN 1) Kaiser Foundation Research Institute, Richmond, Calif., U.S.A. The sex ratio in axenic cultures of P. redivivus was changed from 1 : 1 to a high predominance of males by imposing a stress of low protein supplement and high temperature, 28° C. The females developed normally after removal of the stress. Previous reports have indicated that the sex ratio of certain nematodes vanes during the year, perhaps as a function of environmental changes (Triantaphyllou, 1960). Most of these reports have been based on field observations. Working with axenic culture, we have been able to show a variation in the sex ratio in the dioecious nematode, Panagrellus redivivus (L.). This organism is similar in its nutritional requirements to the hermaphroditic nematode, Caenorhabditi.r brigg.rae (Hansen, Buecher & Yarwood, 1964). A combination of low protein and high temperature, used to provide stress conditions, was found to be inhibitory to growth of P. redivivus females. Methods Cultures were initiated with new larvae of P. redivivu.r which were obtained from gravid females that had been washed through several dishes of
Nematologica – Brill
Published: Jan 1, 1966
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