SHORT COMMUNICATIONS †C. NETSCHER1) & R. DUPONNOIS2): Use of aqueous suspensions for storing and inoculating spores of Pasteuria penetrans, parasite of Meloidogyne spp. Maintaining the hyperparasite, Pasteuria penetrans (Thorne, 1940), on Meloi- dogyne spp. is time consuming because of low rates of infection of females when spore-carrying juveniles are inoculated onto tomato plants. This paper describes techniques to increase spore concentrations and experiments on the effective- ness and longevity of spores. A simple sterilisation technique for equipment is described, and the practical value of the work is outlined. A population (MiKT) of M. incognita Kofoid & Chitwood isolated from to- bacco (Nicotiana tabacum L.) growing in Kali Tirto, Yogyakarta, Indonesia and maintained on tomato (Lycopersicon esculentum L. cv. Intan) was used in all studies. Juveniles of MiKT were inoculated with spores of P. penetrans (Stirling & Wachtel) isolated from adult females of M. incognita dissected from roots of tomato plants from Klarangan, Yogyakarta. This population was designated as PpKL. Juveniles of MiKT encumbered with spores of PpKL were inoculated at 1000 juveniles per pot to four-week-old tomato plants growing in 100 cm3 pots of sterile sandy loam in a glasshouse (30°C day, 25°C night). Eight weeks after inoculation,
Nematologica – Brill
Published: Jan 1, 1998
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