The response of five round-cyst nematodes (Heteroderidae) to five artificial hatching agents

The response of five round-cyst nematodes (Heteroderidae) to five artificial hatching agents 363 D. N. GREET1 : The response of five round-cyst nematodes (Heteroderidae) to five artificial hatching agents. In attempts to hatch the eggs of round-cyst nematodes, (Heterodera, sub. gen. Globodera) five artificial hatching agents were tried which Clarke and Shepherd (1965) found effective for other Heteroderidae. Differences in response are reported which might conceivably be used to differentiate species. The cysts of H. rostochiensis, H. pallida, H. solanacearum, H. tabacum and H. virginiae used in the tests had been stored air dried at room temperature for 2 years. Seven batches of three 100-cyst replicates were placed in separate watch glasses and soaked in distilled water at 20° C. After a week the water and any hatched larvae were removed and replaced either by water or by solutions of six hatching agents (Table I). Hatched larvae were removed and counted and solutions renewed every week for the next five weeks. The concentrations of artificial hatching agents were those found best by Shepherd & Clarke (1965). Root diffusate of Solanum dulcamara, a host common to all five nematode species, was obtained by collecting leachates from six pots each containing a well grown plant. Accumulative totals were determined for each treatment Nematologica Brill

The response of five round-cyst nematodes (Heteroderidae) to five artificial hatching agents

Nematologica , Volume 20 (3): 363 – Jan 1, 1974

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© 1974 Koninklijke Brill NV, Leiden, The Netherlands
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