Haematologia , Vol. 32, No. 3, pp. 239– 252 (2002) Ó VSP 2002. Also available online - www.vsppub.com Article The involvement of the Na C / H C exchanger in the formation of microvesicles by porcine platelets HALINA STELMACH, TOMASZ RUSAK and MARIAN TOMASIAK ¤ Department of Physical Chemistry, Medical Academy of Bialystok, Mickiewicza 2A, 15-230 Bialystok, Poland Abstract —Activated platelets release microvesicles, which express procoagulant activity. The mechanism by which vesicles are formed is not entirely clear. This study was undertaken to determine whether a link exists between the operation of the plasma membrane Na C /H C exchanger (NHE) and vesiculation. It was found, that platelets treated with NHE-simulating monensin and the sodium in ux-inducing gramicidin (without concomitant H C ef ux) produced vesicles demonstrating procoagulant activity. Alkalinization of platelet cytosol by NH 4 Cl failed to evoke vesicle release. Collagen and phorbol ester (PMA)-evoked vesiculation was diminished in the presence of 5-( N - ethyl- N -isopropyl amiloride) (EIPA, inhibitor of NHE) or GF 109203X (inhibitor of protein kinase C). Vesicle formation induced by collagen, PMA, and the calcium ionophore A23187 was less pronounced in the absence of external Na C . In comparison with
Haematologia – Brill
Published: Jan 1, 2002
Keywords: porcine platelets; platelet microparticles.; procoagulant activity; Na/H exchanger
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