STUDIES ON THE CULTURING OF CERTAIN ECTOPARASITIC NEMATODES ON PLANT CALLUS TISSUE 1) BY S. KHERA AND B. M. ZUCKERMAN Cranberry Experiment Station, University of Massaschusetts, East Wareham, Mass., U.S.A. Tylenchus agricola and Tylenchorhynchus claytoni reproduced rapidly on alfalfa callus grown under sterile conditions on a nutrient medium. Attempts to propagate these nematodes on tissue of six other plants under the same conditions were negative as were attempts to treat five other nematodes in the same manner. Krusberg (1961) reported the propagation of several plant parasitic nematodes on alfalfa callus tissue grown on a nutrient medium containing 2, 4 dichlorophen- oxyacetic acid (2,4-D). In these studies Dityleizchui- dipsaci (K3hn) and A phe- lenchoide.r ritzenaabo.ri Schwartz reproduced rapidly, Pratylenchus zeae Graham and P. penetran.r (Cobb) reproduced less rapidly, and Tylenchorhyiichus capitatu.r Allen and Ho plolaimu.r coronatus Cobb very slowly on callus. Krusberg also re- ported unsuccessful culture on tissues of carrot, sweet potato, tobacco, tomato and soybean. Similarly, attempts to culture Ditylenchu.r tYZ f or??2Z.r Hirschmann & Sasser, Meloidogyne incognita acrita Chitwood, Helicotylencbus nannus Steiner and a Trichodorus sp. also failed. This paper reports attempts to apply these methods to the culture of other plant parasitic nematodes on several
Nematologica – Brill
Published: Jan 1, 1962
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