Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage

Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage Nematology , 2004, Vol. 6(6), 911-917 Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage Paul F.L. F ITTERS ∗ and Christine T. G R I FFI N Institute of Bioengineering and Agroecology and Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland Received: 24 September 2004; revised: 5 November 2004 Accepted for publication: 5 November 2004 Summary – Infective juveniles (IJ) of three Heterorhabditis megidis isolates, HF85, EU17 and UK211, were stored in water at 20 ◦ C for up to 10 weeks. At 2-week intervals, activity, infectivity, energy reserves and survival were measured. There was no difference between the three isolates in infectivity, which increased significantly over the first 2 weeks and declined gradually thereafter. IJ became inactive during storage. Out of storage, the highest activity was recorded in week 0: nearly all IJ were active within the first minute of observation and remained active for the 20-min observation period. With increasing storage time, an increasing proportion of IJ were inactive in the first minute, reaching 83-96% by week 6. The time taken by 50% of the IJ to become active (AT 50 ) initially increased with nematode age, reaching a maximum http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage

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Publisher
Brill
Copyright
© 2004 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1388-5545
eISSN
1568-5411
D.O.I.
10.1163/1568541044038597
Publisher site
See Article on Publisher Site

Abstract

Nematology , 2004, Vol. 6(6), 911-917 Spontaneous and induced activity of Heterorhabditis megidis infective juveniles during storage Paul F.L. F ITTERS ∗ and Christine T. G R I FFI N Institute of Bioengineering and Agroecology and Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland Received: 24 September 2004; revised: 5 November 2004 Accepted for publication: 5 November 2004 Summary – Infective juveniles (IJ) of three Heterorhabditis megidis isolates, HF85, EU17 and UK211, were stored in water at 20 ◦ C for up to 10 weeks. At 2-week intervals, activity, infectivity, energy reserves and survival were measured. There was no difference between the three isolates in infectivity, which increased significantly over the first 2 weeks and declined gradually thereafter. IJ became inactive during storage. Out of storage, the highest activity was recorded in week 0: nearly all IJ were active within the first minute of observation and remained active for the 20-min observation period. With increasing storage time, an increasing proportion of IJ were inactive in the first minute, reaching 83-96% by week 6. The time taken by 50% of the IJ to become active (AT 50 ) initially increased with nematode age, reaching a maximum

Journal

NematologyBrill

Published: Jan 1, 2004

Keywords: ENTOMOPATHOGENIC NEMATODE; PHASED INFECTIVITY; ACTIVITY; SURVIVAL; STARVATION

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