SOME OBSERVATIONS ON MOULTING IN CAENORHABDITIS ELEGANS BY R. N. SINGH * and J. E. SULSTON MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England Moulting of Caenorhabditis elegans has been observed by Nomarski interference contrast micro- scopy, and by electron microscopy of animals at selected stages. The wild type, cell division mutants and animals in which cells had been ablated by a laser microbeam were examined. The median lateral hypodermis, or "seam", is required for the formation of alae and for dauer larva maturation. During cuticle deposition, large Golgi bodies are seen in the seam cells. The excretory system is not essential for moulting. Using the technique described by Sulston & Horvitz (1977), it is possible to observe a single specimen of Caenorhabditi.r elegan.r throughout its life cycle, with- out the use of anaesthetics, by high resolution Nomarski interference contrast microscopy. Thus, events can be followed in their natural succession and animals can be removed at any stage for electron microscopic examination. A laser micro- beam system and genetic methods allow experimental manipulation of the organism. In describing moulting, we shall be concerned to a great extent with the hypo- dermis, which both generates and
Nematologica – Brill
Published: Jan 1, 1978
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