Restraining small decapods and amphipods for in vivo laboratory studies

Restraining small decapods and amphipods for in vivo laboratory studies During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Crustaceana Brill

Restraining small decapods and amphipods for in vivo laboratory studies

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Publisher
Brill
Copyright
Copyright © Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0011-216x
eISSN
1568-5403
D.O.I.
10.1163/15685403-00003778
Publisher site
See Article on Publisher Site

Abstract

During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.

Journal

CrustaceanaBrill

Published: Mar 30, 2018

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