Nematology , 2000, Vol. 2(7), 719-731 Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags James M C C ARTER 1 , Pierre A BAD 2 , John T. J ONES 3 and David B IRD 4, * 1 Genome Sequencing Center, Department of Genetics, Box 8501, Washington University School of Medicine, St. Louis, MO 63108, USA 2 Unité Santé des Plantes et Environnement, INRA, 123, Bd Francis Meilland, B.P. 2078, 06606, Antibes, France 3 Mycology, Bacteriology and Nematology Unit, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK 4 Plant Nematode Genetics Group, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA Accepted for publication: 16 October 2000 Summary – Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionisegene discovery, as well as aiding in genome physical mapping and expression pro ling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how
Nematology – Brill
Published: Jan 1, 2000
Keywords: GLOBODERA; NEMAGENE; CLUSTERING; MELOIDOGYNE; EST; CAENORHABDITIS ELEGANS
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