Nematology , 2003, Vol. 5(6), 859-864 Puri cation of two substances from bulbs of onion ( Allium cepa L.) with nematicidal activity against Meloidogyne exigua Goeldi Daniel Ru no A MARAL 1 , Fernanda Emanuele da R OCHA O LIVEIRA 3 , Denilson Ferreira O LIVEIRA 1 ; ¤ and Vicente Paulo C AMPOS 2 1 Departamento de Química & 2 Departamento de Fitopatologia,Universidade Federal de Lavras, CP 37, CEP 37200-000, Lavras (MG), Brazil 3 Centro Universitário de Lavras, Lavras (MG), Brazil Received: 10 January 2003; revised: 3 September 2003 Accepted for publication: 3 September 2003 Summary – To contribute to the development of a new method for Meloidogyne exigua Goeldi control, a nematode widely disseminated through Brazilian coffee elds, this work aimed to purify the nematicidal substances produced by onion ( Allium cepa L.). Thus, the methanolic extract of onion bulbs was concentrated under reduced pressure and submitted to a puri cation process guided by in vitro assays with second-stage juveniles (J2) of M. exigua . After several steps, two nematicidal substances that seemed to be interconverting when in solution were isolated. When exposed to 500 ppm aqueous solutions of substances 1 and 2, 55 and
Nematology – Brill
Published: Jan 1, 2003
Keywords: CHROMATOGRAPHY; ROOT-KNOT NEMATODE; IN VITRO ASSAY; NEMATICIDE
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