Purification of two substances from bulbs of onion (Allium cepa L.) with nematicidal activity against Meloidogyne exigua Goeldi

Purification of two substances from bulbs of onion (Allium cepa L.) with nematicidal activity... Nematology , 2003, Vol. 5(6), 859-864 PuriŽ cation of two substances from bulbs of onion ( Allium cepa L.) with nematicidal activity against Meloidogyne exigua Goeldi Daniel RuŽ no A MARAL 1 , Fernanda Emanuele da R OCHA O LIVEIRA 3 , Denilson Ferreira O LIVEIRA 1 ; ¤ and Vicente Paulo C AMPOS 2 1 Departamento de Química & 2 Departamento de Fitopatologia,Universidade Federal de Lavras, CP 37, CEP 37200-000, Lavras (MG), Brazil 3 Centro Universitário de Lavras, Lavras (MG), Brazil Received: 10 January 2003; revised: 3 September 2003 Accepted for publication: 3 September 2003 Summary – To contribute to the development of a new method for Meloidogyne exigua Goeldi control, a nematode widely disseminated through Brazilian coffee Ž elds, this work aimed to purify the nematicidal substances produced by onion ( Allium cepa L.). Thus, the methanolic extract of onion bulbs was concentrated under reduced pressure and submitted to a puriŽ cation process guided by in vitro assays with second-stage juveniles (J2) of M. exigua . After several steps, two nematicidal substances that seemed to be interconverting when in solution were isolated. When exposed to 500 ppm aqueous solutions of substances 1 and 2, 55 and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematology Brill

Purification of two substances from bulbs of onion (Allium cepa L.) with nematicidal activity against Meloidogyne exigua Goeldi

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Publisher
BRILL
Copyright
© 2003 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1388-5545
eISSN
1568-5411
D.O.I.
10.1163/156854103773040763
Publisher site
See Article on Publisher Site

Abstract

Nematology , 2003, Vol. 5(6), 859-864 PuriŽ cation of two substances from bulbs of onion ( Allium cepa L.) with nematicidal activity against Meloidogyne exigua Goeldi Daniel RuŽ no A MARAL 1 , Fernanda Emanuele da R OCHA O LIVEIRA 3 , Denilson Ferreira O LIVEIRA 1 ; ¤ and Vicente Paulo C AMPOS 2 1 Departamento de Química & 2 Departamento de Fitopatologia,Universidade Federal de Lavras, CP 37, CEP 37200-000, Lavras (MG), Brazil 3 Centro Universitário de Lavras, Lavras (MG), Brazil Received: 10 January 2003; revised: 3 September 2003 Accepted for publication: 3 September 2003 Summary – To contribute to the development of a new method for Meloidogyne exigua Goeldi control, a nematode widely disseminated through Brazilian coffee Ž elds, this work aimed to purify the nematicidal substances produced by onion ( Allium cepa L.). Thus, the methanolic extract of onion bulbs was concentrated under reduced pressure and submitted to a puriŽ cation process guided by in vitro assays with second-stage juveniles (J2) of M. exigua . After several steps, two nematicidal substances that seemed to be interconverting when in solution were isolated. When exposed to 500 ppm aqueous solutions of substances 1 and 2, 55 and

Journal

NematologyBrill

Published: Jan 1, 2003

Keywords: CHROMATOGRAPHY; ROOT-KNOT NEMATODE; IN VITRO ASSAY; NEMATICIDE

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