157 suitable rhizosphere soil have been satisfactorily processed using 35 ml sucrose solution in 50 ml centrifuge tubes, yielding eggs of T. viruliferus and, when present, those of a Paratylenchns sp. After extraction by this technique, eggs of all genera developed normally during embryogenic studies. CAVENESS, F. E. & JENSEN, H. J. (1935). Modification of the centrifugal-flotation technique for the isolation and concentration of nematodes and their eggs from soil and plant tissue. Proc. helminth. Soc. Wash., 22, 87-89. PITCHER, R. S. & FLEGG, J. J. M. (1965). Observation of root feeding by the nematode Trichodorus viruliferus Hooper. Nature, Lond., 207, 317. SHORT COMMUNICATIONS Pramila GUPTA & Gopal SWARUP 1): Occurrence of living adult males and second stage larvae inside live adult females of Anguina tritici. During investigations on the ear-cockle disease of wheat, the authors came across a few females carrying either 1-6 second stage larvae alone or along with a single live adult male inside their bodies (Figs. 1 & 2). Larvae and males moved actively in the body cavity and independently of each other within the females, sometimes turning the head towards the head of the female and at other times towards her tail. The females were fully developed and sometimes contained eggs in advanced stages of development within the uterus. They moved with the movement of the larvae and/or males present inside. The living condition of the females was confirmed by the observation of muscular movements in the oesophagus. Females with larvae or males or both inside remained alive for 30 days under laboratory con- ditions (in a humid chamber at a temperature of 10 ± 4° C) after which observations were discontinued. The movements were very slow at the end of the period and during this time no eggs or larvae were released outside the female body. The individuals were obtained from experimental plots where wheat was sown on 1st and 15th November; ear-cockle infection was 53 and 74 per cent in the two sowings respectively. In about 400 galls 35 females were observed to contain 2nd stage larvae, varying in numbers from 1-6; 9 females had only males inside; and 3 females had both males and larvae inside their bodies. The atmospheric temperature from seeding to the heading stage ranged between 10-20° C. Hatching of eggs and emergence of larvae while within the female uterus is known in the animal parasitic nematodes and in some members of Rhabditida (Hirschmann in Sasser & Jenkins, 1960). However this phenomenon does not seem to be known for a plant parasitic nematode. Moreover occurrence of adult males within the female body appears to be an unknown phenomenon. At present we cannot explain how the males got into the females, whether they developed from second stage larvae without feeding, or entered secondarily through the genital pore of the female. Possible explanations for such a peculiarity could be that temperature has an important bearing on the activity of the female in releasing eggs or that such females had become too old to discharge eggs. REFERENCE HIRSCHMANN, H. (1960). Reproduction of nematodes. Nematology, edited by J. N. Sasser & W. R. Jenkins pp. 140-146. Univ. of N. Carolina Press, Chapel Hill. 1) Division of Nematology, Indian Agricultural Research Institute, New Delhi, India.
Nematologica – Brill
Published: Jan 1, 1968
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera