NON-SPECIFIC ESTERASE VARIATION IN FIELD POPULATIONS OF THE POTATO CYST NEMATODES GLOBODERA ROSTOCHIENSIS AND G. PALLIDA BY P. C. FOX1) and H. J. ATKINSON Department of Pure and Applied Zoology, The University of Leeds, Leeds LS2 9JT, England Non-specific esterase (E.C.18.104.22.168) polymorphism in reference pathotypes of potato cyst nematodes (PCN) was studied by isoelectric focusing (IEF) and used to compare pathotyped field populations of PCN. Agarose IEF, pH 3-10, produced the best separation of discriminatory isoenzymes, and a distinct difference was detected between the two species of PCN with G. rostochiensis possessing an cxtra band. Six field populations of G. rostochiensis Ro1 and one population of G. pallida also possessed this band, but the band was absent from 21 other populations of G. pallida with varying pathotype composition. The usefulness of this character in the rapid identification of field populations of PCN is discussed. Keywords: identification, pathotypes, integrated control, biochemical taxonomy, isoelectric focusing. The two species of potato cyst nematode (PCN), Globodera pallida and G. rostochiensi.s are important crop pests in potato growing areas worldwide. Rapid identification of PCN pathotypes in infested fields is needed if maximum benefit in integrated control is to be obtained from new potato
Nematologica – Brill
Published: Jan 1, 1988
Keywords: biochemical taxonomy; isoelectric focusing; integrated control; pathotypes; identification
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