Nematology , 2002, Vol. 4(3), 445-447 Short communication New process for preparing heteroderid cysts for scanning electron microscopy Paola L AX and Marcelo E. D OUCET ¤ Scanning electron microscopy (SEM) is an important tool in morphological and taxonomic studies of plant- parasitic nematodes (Wergin, 1981; Eisenback, 1986). Indeed, identi cation of species of cyst nematodes in the family Heteroderidae is based mainly on analysis of the external (pattern of cuticle and vulval region) and internal morphology (bullae, underbridge) of cysts (Green, 1975; Mulvey & Golden, 1983). Numerous particles, principally of mineral origin, are often found attached to the external wall of cysts that are extracted from the soil. Particles which are probably from the internal content of the cyst are often found on the inside wall near the vulval region. These artifacts can obstruct morphological studies conducted with SEM. Techniques for processing whole cyst nematodes (San- tos, 1994) and vulval cones (Hirschmann & Triantaphyl- lou, 1979) have been described which include in ltrating the specimens with pure glycerin or solutions of alcohol- glycerin. The material was processed for ca 3 days and 1 week, respectively. Processing the interior of the vul- val cone for SEM has been
Nematology – Brill
Published: Jan 1, 2002
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera