MASS PRODUCTION OF THE ENDOPARASITIC FUNGI DRECHMERIA CONIOSPORA, VER TICILLIUM BALANOIDES AND HARPOSPORIUM ANGUILLULAE IN LIQUID CULTURE BY U. LOHMANN and R. A. SIKORA Institut für Pflanzenkrankheiten der Universität Bonn, Nußallee 9, 5300 Bonn 1, F.R. Germany Mycclial growth and rate of sporulation of Drechmeria coniospora, Verticillium balanoides and Har- posporium anguillulae were tested in different concentrations on liquid corn meal (CM) and on potato dextrose (PD) media. Mycelial growth of all three fungi was greatest in PD, whereas conidial production was highest in CM. Spore productivity increased in CM and decreased in PD with increasing nutrient levels. Mycelial production was greatest at high nutrient concentrations of both media. Of the three fungi, the highest levels of growth and sporulation were achieved by V. balanoides. Conidia of the endoparasitic fungi produced in liquid culture retained their parasitic capabilities. Keywords: Nernatophagous fungi, liquid culture, biological control, culture methods. The endoparasitic fungi, Drechmeria coniospora (Drechsler) Gams and Jansson (basionym Meria coniospora Drechsler) and Hirsutella heteroderae Sturhan and Schneider have been shown to control plant parasitic nematodes in greenhouse experiments (Jansson et al., 1985; Juhl, 1985). The absence of large scale pro- duction methods for these organisms is a major drawback
Nematologica – Brill
Published: Jan 1, 1989
Keywords: liquid culture; culture methods; Nernatophagous fungi; biological control
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