Nematology , 2009, Vol. 11(5), 649-668 ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species Wolfgang B URGERMEISTER 1 , ∗ , Helen B R A A S C H 2 , Kai M ETGE 3 , Jianfeng G U 4 , Thomas S CHRÖDER 5 and Elvira W OLDT 1 1 Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn Institute, Messeweg 11, 38104 Braunschweig, Germany 2 Kantstrasse 5, 14471 Potsdam, Germany 3 Institute for Biosafety of Genetically Modified Plants, Julius Kühn Institute, Erwin Baur Strasse 27, 06484 Quedlinburg, Germany 4 Ningbo Entry-Exit Inspection and Quarantine Bureau, 9 Mayuan Road, Ningbo, Zhejiang, China 5 Institute for National and International Plant Health, Julius Kühn Institute, Messeweg 11, 38104 Braunschweig, Germany Received: 1 September 2008; revised: 11 November 2008 Accepted for publication: 12 November 2008 Summary – Restriction analysis of amplified ribosomal ITS sequences has provided species-specific fragment patterns for nematodes of several genera, including Bursaphelenchus . We used restriction enzymes Rsa I, Hae III, Msp I, Hin fI and Alu I to produce ITS-RFLP reference profiles of 44 Bursaphelenchus species, including two intraspecific types in each of B. mucronatus and B. leoni . In addition, reference profiles of
Nematology – Brill
Published: Jan 1, 2009
Keywords: SPECIES IDENTIFICATION; INTRASPECIFIC TYPES; PINE WOOD NEMATODE (PWN); MOLECULAR DIAGNOSIS; ITS MICROHETEROGENEITY
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