ESTIMATE OF THE NUMBER OF INFECTIVE LARVAE OF NEOAPLECTANA CARPOCAPSAE (NEMATODA: STEINERNEMATIDAE) IN A SOIL SAMPLE BY ZDENEK MRÁ � EK Institute of Entomology, Department of Insect Pathology, Czechoslovak Academy of Sciences, 370 05 � eské Bud � jovice, Czechoslovakia Using Galleria traps, the number of infective larvae of Neoaplectana carpocapsae in soil samples was estimated. Sterile soil samples, in Galleria traps, were inoculated with different concentra- tions of infective larvae, from 10 to 5 × 105, and the concentration was related to the percentage of dead larvae of Galleria mellonella and the rate of mortality. The results suggest that using our method, an estimate can be made of the number of infective larvae in soil samples. Keywords: Neoaplectana carpocapsae, insect parasitic nematodes, techniques. Bedding & Akhurst (1975) and later Mracek (1980) developed methods for isolating entomopathogenic nematodes from soil samples by placing insect baits (larvae of Galleria mellonella) in soil. These methods have been investigated by Bovien (1937) and Weiser & Koehler (1955). Using this technique (Galleria traps) to investigate the distribution of steinernematids in Czechoslovakia, Mracek (1980) observed that the number of infested G. mellonella larvae in the trap varied: he presumed that the percentage of
Nematologica – Brill
Published: Jan 1, 1982
Keywords: Neoaplectana carpocapsae; insect parasitic nematodes; techniques
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