Erratum

Erratum 571 After staining in boiling lactophenol-cotton blue, roots are washed in water to remove excess stain. They are then placed in an acid solution comprising 1 g zinc chloride per 1.7 ml of 12N hydrochloric acid (Holloway & Baker, 1968) for destaining. Time of destaining varies with temperature, depth of staining, root thickness, and the purpose for which the acid treatment is required. For example, to count nematodes directly the roots are left longer in the acid solution than if the aim is merely to soften the roots to allow the nematodes to be dissected out. In the acid solution, the plant tissues, the cortex first, soften, the cells separate and the stain is removed simultaneously. With time, all the cells of the root destain, and finally break down. Nematodes eventually destain, swell and 'burst'; but are more resistant and hence take longer for each stage of the process. The optimum temperature for differentiation of Heterodera avenae in cereal roots is between 25 and 30° C. At temperatures above 30°, the nematodes de-stain and 'burst' after about 15 min. exposure to the acid solution, but this process takes several hours at 20°. At 30°, nematodes in young thin roots can be counted directly from roots while still in the acid solution, 10-20 min after immersion. Overstained roots and older, thicker roots take proportionately longer to de-stain. The process can be hastened by gently squashing the roots before immersion in the acid solution. HOLLOWAY, P. J. & BAKER, E. A. (1968). Isolation of cuticles with zinc chloride-hydrochloric acid solution. Pl. Physiol. 43, 1878-1879. ERRATUM In the paper by M. R. Siddiqi: Systematics of the genus Trichodorus, Nematologica 19: 259-278: 1. P. 259, line 19 : Diptherophora should be Diphtherophora. 2. P. 275, line 22: tanssaniensis should be tansaniensis. 3. P. 278, lines 35 and 43: Stationary should be Stationery. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

Erratum

Nematologica , Volume 19 (4): 571 – Jan 1, 1973
Free
1 page

Loading next page...
1 Page
 
/lp/brill/erratum-JFw0FaHR7J
Publisher
Brill
Copyright
© 1973 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
D.O.I.
10.1163/187529273X00673
Publisher site
See Article on Publisher Site

Abstract

571 After staining in boiling lactophenol-cotton blue, roots are washed in water to remove excess stain. They are then placed in an acid solution comprising 1 g zinc chloride per 1.7 ml of 12N hydrochloric acid (Holloway & Baker, 1968) for destaining. Time of destaining varies with temperature, depth of staining, root thickness, and the purpose for which the acid treatment is required. For example, to count nematodes directly the roots are left longer in the acid solution than if the aim is merely to soften the roots to allow the nematodes to be dissected out. In the acid solution, the plant tissues, the cortex first, soften, the cells separate and the stain is removed simultaneously. With time, all the cells of the root destain, and finally break down. Nematodes eventually destain, swell and 'burst'; but are more resistant and hence take longer for each stage of the process. The optimum temperature for differentiation of Heterodera avenae in cereal roots is between 25 and 30° C. At temperatures above 30°, the nematodes de-stain and 'burst' after about 15 min. exposure to the acid solution, but this process takes several hours at 20°. At 30°, nematodes in young thin roots can be counted directly from roots while still in the acid solution, 10-20 min after immersion. Overstained roots and older, thicker roots take proportionately longer to de-stain. The process can be hastened by gently squashing the roots before immersion in the acid solution. HOLLOWAY, P. J. & BAKER, E. A. (1968). Isolation of cuticles with zinc chloride-hydrochloric acid solution. Pl. Physiol. 43, 1878-1879. ERRATUM In the paper by M. R. Siddiqi: Systematics of the genus Trichodorus, Nematologica 19: 259-278: 1. P. 259, line 19 : Diptherophora should be Diphtherophora. 2. P. 275, line 22: tanssaniensis should be tansaniensis. 3. P. 278, lines 35 and 43: Stationary should be Stationery.

Journal

NematologicaBrill

Published: Jan 1, 1973

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off