SHORT COMMUNICATIONS J. KIMPINSKI 1) & H. E. WELCH 2) : Comparison of Baermann funnel and sugar flotation extraction from compacted and non-compacted soils. Sampling and extraction techniques were important factors in studying nematode populations in western Canadian soils. A known number of a mixed population of living soil nematodes was inoculated into 50 g of autoclaved clay (12.1% sand, 39.2Vo silt, 48.7% clay) and of sand (98.3% sand, 0.7% silt, 1.0% clay) to compare the extraction efficiency of the Baermann funnel (BF) and of the sugar flotation (SF) techniques. In the BF technique the soil was washed through a 40 and a 325 mesh screen. The residue from the 325 mesh screen was placed in a BF consisting of a 15.3 cm glass funnel and two layers of facial tissue supported by loosely woven muslin. Soil was left in the funnel for 24 hours at 23°C. One hour prior to draining, the water was swirled to dislodge nematodes attached to slanted portions of the funnel. The SF technique was similar to the Centrifugal Flotation technique of Caveness and Jensen (1955). A 40 and a 325 mesh screen were used to wash the 50 g soil samples, and
Nematologica – Brill
Published: Jan 1, 1971
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