CHANGES OF THYROTROPIN-RELEASING HORMONE (TRH) LEVELS IN BRAIN REGIONS AND PITUITARY DURING INDUCED METAMORPHOSIS OF AMBYSTOMA MEXICANUM by B. DE GROEF1, V.M. DARRAS1, L. ARCKENS2, H.H.J. GERETS2, E.R. KÜHN1 and K.L. GERIS1 (1 Laboratory of Comparative Endocrinology, K. U.Leuven, Naamsestraat 61, B-3000 Leuven, Belgium; 2Laboratory of Neuroendocrinology and Immunological Biotechnology, K. U.Leuven, Naamsestraat 59, B-3000 Leuven, Belgium. Email: firstname.lastname@example.org) ABSTRACT Axolotls (Ambystoma mexicanum) are neotenic amphibians: they keep larval features during their entire lifetime. However, metamorphosis can be induced by injection of thyroid hormones or thyrotropin (TSH). The role played by thyrotropin-releasing hormone (TRH) in amphibian metamorphosis is not clear, since TRH treatment never succeeded in inducing metamorphosis in amphibian larvae. We studied the changes of TRH and plasma thyroid hormone levels in axolotls during thyroxine (T4)-induced metamorphosis. TRH levels in skin, hypothalamus, pituitary and extrahypothalamic region of the brain as well as plasma T4 and triiodothyronine (T3) levels were measured by radioimmunoassay (RIA). Preliminary data on the expression of TRH receptors in the pituitary of a neotenic and a metamorphosed animal were obtained by in situ hybridisation with an antisense oligonucleotide probe, based on nucleotides 1011 to 1060 of chicken TRH receptor mRNA. In general, TRH RIA
Netherlands Journal of Zoology (in 2003 continued as Animal Biology) – Brill
Published: Jan 1, 2000
Keywords: TRH; amphibian; pituitary; hypothalamus; TRH receptor; axolotl; metamorphosis
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