CARBOHYDRATE REQUIREMENT OF CAENORHABDITIS ELEGANS AND THE FINAL DEVELOPMENT OF A CHEMICALLY DEFINED MEDIUM BY N. C. LU and K. M. GOETSCH Department of Nutrition and Food Science, San Jose State University, San Jose, CA 95192-0058, U.S.A. The carbohydrate requirement in the free-living nematode Caenorhabditis elegans was investi- gated. Glucose, fructose, sucrose, trehalose and glycogen were tested individually at concentra- tions of 0, 1.3, 6.5, 32.5, and 162.5 mg/ml as the energy source in a chemically defined medium containing C. briggsae Maintenance Medium (CbMM without glucose), 50 µg/ml cytochrome c and 50 µg/ml β -sitosterol. Potassium acetate, used as the energy source in other studies, was not added to the medium. Therefore, carbohydrate was the major energy source for the nematode. At 32.5 mg/ml, glucose was found to support the maximal population at 80,000 nematodes/ml (100%), followed by glycogen (96%) and trehalose (73%). Population was significantly reduced when fructose (46%) or sucrose (26%) was the carbohydrate source. Toxicity was shown at 162.5 mg/ml for four carbohydrates tested, except glycogen. These results suggested that all five carbohydrates can be utilized as energy sources by C. elegans; however, the degree of utilization of each carbohydrate by C. elegans varied. Since
Nematologica – Brill
Published: Jan 1, 1993
Keywords: axenic cultivation; glycogen; glucose; free living nematode; energy source
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