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To date, seven Bursaphelenchus species have been reported in surveys of the Czech Republic (Čermák et al. , 2013). However, the occurrence of some species, such as Bursaphelenchus hofmanni Braasch, 1998, is limited to single detections in imported coniferous wood (unpubl. data in Braasch, 2001). During a survey to determine pathogenic agents on hops ( Humulus lupulus L.) conducted by State Phytosanitary Administration (CZ) in hops fields and nurseries in 2012, B. hofmanni was found in a mixture of peat and soil in a hops seedling nursery in Žatec (Bohemia, Czech Republic). The occurrence of Bursaphelenchus species in non-woody plants or substrates has been previously reported for species of the fungivorus group, such as B. gonzalezi Loof, 1964, B. hunti Giblin & Kaya, 1983 and B. fungivorus Franklin & Hooper, 1962. Species belonging to the hofmanni group are often associated with wood products such as packaging material (Gu et al. , 2006) and, to our knowledge, this is the first time that this species has been found associated with peat substrate and soil. Nematodes were isolated from 60 g of peat substrate (3 parts peat to 1 part soil) associated with hops seedlings, and extracted using the Baermann
Nematology – Brill
Published: Jul 18, 2014
Keywords: Aphelenchoididae; detection survey; Humulus lupulus ; ITS-RFLP patterns; molecular; morphology; point mutations; restriction enzymes
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