Black mulberries (Morus nigra) as a natural dye for animal tissues staining

Black mulberries (Morus nigra) as a natural dye for animal tissues staining Animal Biology 61 (2011) 49–56 brill.nl/ab Black mulberries ( Morus nigra ) as a natural dye for animal tissues staining Ehab Tousson 1 , ∗ and Bahija Al-Behbehani 2 1 Department of Zoology, Faculty of Science, Tanta University, Egypt 2 Science Department, College of Basic Education, PAAET, Kuwait Abstract Natural dyes produce an extraordinary diversity of rich and complex colours as well as unexpected results, making them exciting to use. Natural dyes have been used for staining wool, silk, carpet and cotton. Black mulberry ( Morus nigra ) has strong staining activity and a distinct flavor with juicy and acidic characteristics making them attractive for use in the processing industry in products such as fruit juice, ice cream, jelly, and jam. Aim of this study was to investigate a new staining method using black mulberry for whole mount and transverse sections staining of fasciola. Adult liver flukes ( Fasciola sp.) were collected from the livers of naturally infected cows at slaughterhouse, washed with physiological saline solution. Some adult Fasciola were collected, immersed in 10% neutral buffered formalin for fixation, and embedded in paraffin for histological studies. The rest of whole mount of adult worms were collected, and then http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Biology Brill

Black mulberries (Morus nigra) as a natural dye for animal tissues staining

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Publisher
Brill
Copyright
© 2011 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
1570-7555
eISSN
1570-7563
D.O.I.
10.1163/157075511X554419
Publisher site
See Article on Publisher Site

Abstract

Animal Biology 61 (2011) 49–56 brill.nl/ab Black mulberries ( Morus nigra ) as a natural dye for animal tissues staining Ehab Tousson 1 , ∗ and Bahija Al-Behbehani 2 1 Department of Zoology, Faculty of Science, Tanta University, Egypt 2 Science Department, College of Basic Education, PAAET, Kuwait Abstract Natural dyes produce an extraordinary diversity of rich and complex colours as well as unexpected results, making them exciting to use. Natural dyes have been used for staining wool, silk, carpet and cotton. Black mulberry ( Morus nigra ) has strong staining activity and a distinct flavor with juicy and acidic characteristics making them attractive for use in the processing industry in products such as fruit juice, ice cream, jelly, and jam. Aim of this study was to investigate a new staining method using black mulberry for whole mount and transverse sections staining of fasciola. Adult liver flukes ( Fasciola sp.) were collected from the livers of naturally infected cows at slaughterhouse, washed with physiological saline solution. Some adult Fasciola were collected, immersed in 10% neutral buffered formalin for fixation, and embedded in paraffin for histological studies. The rest of whole mount of adult worms were collected, and then

Journal

Animal BiologyBrill

Published: Jan 1, 2011

Keywords: TRANSVERSE SECTION; WHOLE MOUNT STAINING; NATURAL DYES; BLACK MULBERRY; LIVER FLUKES

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