An improved technique for preparing perineal patterns of Meloidogyne spp

An improved technique for preparing perineal patterns of Meloidogyne spp 268 The difference between (3, 4) and (3', 4') is in most cases extremely small, less than one nematode for a larger than 2. In Table Ia the number of nematodes in the supernatant after each of four centrifugations of a given sample is given and their sum S is compared with the calculated number according to (3) S' and according to (4) S". In Table Ib S is obtained after carefully counting the nematodes remaining in the detritus after three centrifugations. The conformity between calculated numbers and numbers observed is in most cases extremely good. Only once is this conformity slightly less (153 calculated against 178 found). This could be due to improper handling as this sample was processed by two persons, but the error is nevertheless far smaller than it would have been without centrifugation. As to the composition of the nematodes collected after each centrifugation, we did not identify species but there is certainly no bias for sizes or weights; some specimens which could be easily recognized showed no marked quantitative difference towards their presence in subsequent centri- fugations. This indicates the possibility that the first supernatant is a representative sample of the whole population http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nematologica Brill

An improved technique for preparing perineal patterns of Meloidogyne spp

Nematologica, Volume 20 (2): 268 – Jan 1, 1974

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Publisher
Brill
Copyright
© 1974 Koninklijke Brill NV, Leiden, The Netherlands
ISSN
0028-2596
eISSN
1875-2926
D.O.I.
10.1163/187529274X00285
Publisher site
See Article on Publisher Site

Abstract

268 The difference between (3, 4) and (3', 4') is in most cases extremely small, less than one nematode for a larger than 2. In Table Ia the number of nematodes in the supernatant after each of four centrifugations of a given sample is given and their sum S is compared with the calculated number according to (3) S' and according to (4) S". In Table Ib S is obtained after carefully counting the nematodes remaining in the detritus after three centrifugations. The conformity between calculated numbers and numbers observed is in most cases extremely good. Only once is this conformity slightly less (153 calculated against 178 found). This could be due to improper handling as this sample was processed by two persons, but the error is nevertheless far smaller than it would have been without centrifugation. As to the composition of the nematodes collected after each centrifugation, we did not identify species but there is certainly no bias for sizes or weights; some specimens which could be easily recognized showed no marked quantitative difference towards their presence in subsequent centri- fugations. This indicates the possibility that the first supernatant is a representative sample of the whole population

Journal

NematologicaBrill

Published: Jan 1, 1974

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