ABSTRACTS OF PAPERS PRESENTED AT THE ANNUAL MEETING OF THE SOCIETY OF NEMATOLOGISTS AT URBANA, ILLINOIS, U.S.A., AUGUST 15-18, 1965 1) N. E. ALGER & J. P. LEHNERT: The counting of Haemonchus contortus L3 larvae by use of a Bausch & Lomb Spectronic 20. A manual microscopic counting method using a gelatin suspension of third stage Haemonchu,r contortus larvae was correlated with a spectrophotometric method. Sample saline suspensions of larvae were read in the spectrophotometer at 600 mp for optical transmittance. The instrument used was a Bausch and Lomb Spectronic 20. Using the optical density (optical density = 2 - log transmittance) as one axis of a graph and the manual count of the same sample as the other axis a straight line graph was obtained. Optical densities from 0.02 mp to 0.84 mp were correlated with the manual counts from less than 100 larvae per ml. to more than 60,000 larvae per ml. An investigator can read a sample of larvae in the spectrophotometer and then read the number of larvae directly from the graph without manual counting. Supported by U. S. Department of Health, Education and Welfare Grant Number AI 05910-01. - Department of Zoology, Ur:i1Jersity
Nematologica – Brill
Published: Jan 1, 1966
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