A MASS CULTURE BIOASSAY METHOD FOR CAENORHABDITIS BRIGGSAE USING POPULATION GROWTH RATE AS A RESPONSE PARAMETER BY CAROLE B. PINNOCK1), W. F. HIEB2) & E. L. R. STOKSTAD1) 1) Nutritional Sciences Department, University of California, Berkeley, California, U.S.A. 2) Biology Department, State University of New York, Buffalo, New York, U.S.A. A bioassay method for C. briggsae using population growth rate as a response parameter is described in which the nematodes are rotated at 1.0 rpm in 5.0 ml medium in 18 mm tubes on a tissue culture rotator. This method permits higher peak populations of nematodes than a previous mass culture method and also allows nephelometric monitoring of population growth. Population growth rate during the initial phase of growth is an effective index of growth and avoids the variations due to inoculum size and accumulation of metabolites associated with use of direct popu- lation counts at fixed time intervals. The use of mass culture for nutrient bioassay using Caenorhabditi.r brigg.rae provides a less variable index of reproduction and is often more convenient than the commonly used larval bioassay method (Lower, Hansen & Yarwood, 1966). Tomlinson & Rothstein (1962) described a method in which nematodes were grown in a
Nematologica – Brill
Published: Jan 1, 1975
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