A FLUIDISING COLUMN FOR EXTRACTING NEMATODES FROM SOIL BY D. L. TRUDGILL*, K. EVANS AND G. FAULKNER Rothamsted Experimental Station, Harpenden, Herts., England An apparatus which uses a controlled water current passing through a sintered plate to separate nematodes from soil particles is described. Nematodes of all types and sizes can be extracted, and the apparatus is particularly useful for extracting white Heterodera females. Construction is simple, so the apparatus is cheap, robust and valuable where only limited facilities are avail- able. It may prove useful for extracting other small soil invertebrates, or their eggs. Methods used to extract nematodes from soil are summarised in Oostenbrink (1960, 1970), Seinhorst (1956), Flegg & Hooper (1970) and Whitehead & Hem- ming (1965). They depend on the ability of nematodes to move through sieves or filters of appropriate mesh, on differences between the physical properties of nematodes and soil particles or on both. Sedimentation and elutriation and then sieving are the most widely used methods, but no single piece of apparatus extracts both cyst and vermiform nematodes efficiently. The apparatus described below uses a controlled water current passing through a sintered plate and extraction depends only on differences in size and
Nematologica – Brill
Published: Jan 1, 1972
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