360 Then (5) cv2(c) = 1 /k + 1 /c per 1 unit of soil If G units are taken (6) CV2(C) = I /G(I/k + 1 /c) Eliminate cv(c) from (3) and (6) (7) cv2(e) = 1 /G[1 /k + 1 /c + cv2(e/c)] Solve G: (8) G = [ 1 /k + 1 /c + cv2(e/c)J /cv2(e) Now choose the cv(e) needed for the experiment. k, cv(e/c) and c should be estimated from presampling, or a best estimate from the literature or one's own experience with other field experiments can be used. For potato cyst nematodes the best estimate of k = 50 for a sample size of I kg soil (Seinhorst, 1988). For cv2(e/c) values ranging from 12 to 20% (mean 16%) are often found, provided that they are estimated from a sufficient number of (small) plots. However, these values may be higher if pesticides are used occasionally or frequently. For free living nematodes the formula can be simplified to: G = [ 1 /k + lie ]lcv2(e) Again, a suitable estimation of k is needed. Meaning of abbreviations used: e eggs or larvae/unit soil G sample size in units of soil c cysts/unit soil
Nematologica – Brill
Published: Jan 1, 1995
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