Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

SEPARATION OF RAT PITUITARY THYROTROPHIC CELLS

SEPARATION OF RAT PITUITARY THYROTROPHIC CELLS A method for the enrichment of live thyrotrophic pituitary cells is described. Pituitary glands of young male rats were removed into Earle's solution and dispersed in a 0·1% trypsin solution containing 0·5% bovine serum albumin, pH 7·2.Nylon fibres (25 μm) were used for the separation of the thyrotrophic cells, by stringing them across a plastic frame which fitted a plastic Petri dish containing the cell suspension. The fibres were washed with light petroleum (b.p. 60–80 °C) and carbon tetrachloride, hydrolysed with 3 m-HCl for 30 min at room temperature and washed with distilled water and phosphate-buffered saline (pH 7·2). The fibres were treated with thyrotrophin releasing hormone (TRH) alone or in the presence of soluble carbodiimide solution. After incubation for 1 h at room temperature, the fibres were transferred to a new Earle's medium and cells were released from the fibres by plucking them with a needle. The separated thyrotrophic cells were identified by radioimmunoassay and by electron microscopy.Using the above-mentioned methods, enrichment of thyrotrophic cells was obtained. Thus, the amounts of TSH, prolactin, LH and GH released, during 2 h of incubation, by 1·5 × 106 unseparated cells were 6·8 ± 0·65, 4·1 ± 0·47, 4·8 ± 0·52 and 5·2 ± 0·68 μg respectively, while the same number of purified thyrotrophic cells released 76·1 ± 0·42, 1·2 ± 0·3, 0·6 ± 0·35 and 1·6 ± 0·22 μg of the same hormones (means ± s.e.m.). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Endocrinology Bioscientifica

SEPARATION OF RAT PITUITARY THYROTROPHIC CELLS

TAL, E.; SAVION, S.; HANNA, N.; ABRAHAM, M.
Journal of Endocrinology , Volume 78 (1) – Jul 1, 1978
Download PDF
7 pages

Loading next page...
 
/lp/bioscientifica/separation-of-rat-pituitary-thyrotrophic-cells-ir4vc4biwA

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Bioscientifica
Copyright
Copyright © 1978 The Authors. All Rights Reserved.
ISSN
0022-0795
eISSN
1479-6805
DOI
10.1677/joe.0.0780141
Publisher site
See Article on Publisher Site

Abstract

A method for the enrichment of live thyrotrophic pituitary cells is described. Pituitary glands of young male rats were removed into Earle's solution and dispersed in a 0·1% trypsin solution containing 0·5% bovine serum albumin, pH 7·2.Nylon fibres (25 μm) were used for the separation of the thyrotrophic cells, by stringing them across a plastic frame which fitted a plastic Petri dish containing the cell suspension. The fibres were washed with light petroleum (b.p. 60–80 °C) and carbon tetrachloride, hydrolysed with 3 m-HCl for 30 min at room temperature and washed with distilled water and phosphate-buffered saline (pH 7·2). The fibres were treated with thyrotrophin releasing hormone (TRH) alone or in the presence of soluble carbodiimide solution. After incubation for 1 h at room temperature, the fibres were transferred to a new Earle's medium and cells were released from the fibres by plucking them with a needle. The separated thyrotrophic cells were identified by radioimmunoassay and by electron microscopy.Using the above-mentioned methods, enrichment of thyrotrophic cells was obtained. Thus, the amounts of TSH, prolactin, LH and GH released, during 2 h of incubation, by 1·5 × 106 unseparated cells were 6·8 ± 0·65, 4·1 ± 0·47, 4·8 ± 0·52 and 5·2 ± 0·68 μg respectively, while the same number of purified thyrotrophic cells released 76·1 ± 0·42, 1·2 ± 0·3, 0·6 ± 0·35 and 1·6 ± 0·22 μg of the same hormones (means ± s.e.m.).

Journal

Journal of Endocrinology – Bioscientifica

Published: Jul 1, 1978

There are no references for this article.