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RNA AND PROTEIN CONTENT IN DIFFERENT HYPOTHALAMIC AREAS: INFLUENCE OF CASTRATION

RNA AND PROTEIN CONTENT IN DIFFERENT HYPOTHALAMIC AREAS: INFLUENCE OF CASTRATION It is generally accepted that the hypothalamus regulates gonadotrophin secretion by means of specific mediators synthesized in the hypothalamus (Martini, Fraschini & Motta, 1968) and usually called releasing factors. Considering that the gonadotrophin releasing factors are probably peptides (Schally, Arimura, Bowers, Kastin, Sawano & Redding, 1968) and that it is possible to demonstrate an alteration in the amount of these substances in the hypothalamus after gonadectomy (Piacsek & Meites, 1966; Watanabe & McCann, 1969), it was of interest to determine the RNA and protein content of the anterior, middle and posterior hypothalamus in normal and in castrated rats.Male albino rats fed on the standard diet of the Instituto de Fisiología and weighing 150–180 g were used. Light and temperature were controlled (25 °C, 12 h light and 12 h darkness). Food and water were available ad libitum. Gonadectomy was performed 6 weeks before the animals were decapitated and the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Endocrinology Bioscientifica

RNA AND PROTEIN CONTENT IN DIFFERENT HYPOTHALAMIC AREAS: INFLUENCE OF CASTRATION

MOGUILEVSKY, J. A.; SCACCHI, P.
Journal of Endocrinology , Volume 51 (1) – Sep 1, 1971
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Publisher
Bioscientifica
Copyright
Copyright © 1971 The Authors. All Rights Reserved.
ISSN
0022-0795
eISSN
1479-6805
DOI
10.1677/joe.0.0510219
Publisher site
See Article on Publisher Site

Abstract

It is generally accepted that the hypothalamus regulates gonadotrophin secretion by means of specific mediators synthesized in the hypothalamus (Martini, Fraschini & Motta, 1968) and usually called releasing factors. Considering that the gonadotrophin releasing factors are probably peptides (Schally, Arimura, Bowers, Kastin, Sawano & Redding, 1968) and that it is possible to demonstrate an alteration in the amount of these substances in the hypothalamus after gonadectomy (Piacsek & Meites, 1966; Watanabe & McCann, 1969), it was of interest to determine the RNA and protein content of the anterior, middle and posterior hypothalamus in normal and in castrated rats.Male albino rats fed on the standard diet of the Instituto de Fisiología and weighing 150–180 g were used. Light and temperature were controlled (25 °C, 12 h light and 12 h darkness). Food and water were available ad libitum. Gonadectomy was performed 6 weeks before the animals were decapitated and the

Journal

Journal of Endocrinology – Bioscientifica

Published: Sep 1, 1971

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