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Expression of growth hormone genes in Atlantic salmon

Expression of growth hormone genes in Atlantic salmon ABSTRACTAtlantic salmon (Salmo salar) possess two genes encoding GH. We have investigated the expression of these two genes in the salmon pituitary. The transcriptional start site was localized 64 nucleotides upstream of the first methionyl codon using primer extension and 5′ specific polymerase chain reaction (PCR) assays. Northern analysis revealed a major Atlantic salmon GH (salGH) transcript band of approximately 1400 nucleotides. As coexpression of the salGH genes is not discernible by transcript length, other techniques were used to assess gene activity; RNase protection analysis revealed GH transcript heterogeneity, while reverse transcription-PCR assays detected transcripts from both genes at approximately equivalent amounts. The encoded salGH protein, generated in vitro and by Escherichia coli, shares electrophoretic and immunoreactive identity with native pituitary salGH. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Endocrinology Bioscientifica

Expression of growth hormone genes in Atlantic salmon

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Publisher
Bioscientifica
Copyright
Copyright © 1993 The Authors. All Rights Reserved.
ISSN
0952-5041
eISSN
1479-6813
DOI
10.1677/jme.0.0110167
Publisher site
See Article on Publisher Site

Abstract

ABSTRACTAtlantic salmon (Salmo salar) possess two genes encoding GH. We have investigated the expression of these two genes in the salmon pituitary. The transcriptional start site was localized 64 nucleotides upstream of the first methionyl codon using primer extension and 5′ specific polymerase chain reaction (PCR) assays. Northern analysis revealed a major Atlantic salmon GH (salGH) transcript band of approximately 1400 nucleotides. As coexpression of the salGH genes is not discernible by transcript length, other techniques were used to assess gene activity; RNase protection analysis revealed GH transcript heterogeneity, while reverse transcription-PCR assays detected transcripts from both genes at approximately equivalent amounts. The encoded salGH protein, generated in vitro and by Escherichia coli, shares electrophoretic and immunoreactive identity with native pituitary salGH.

Journal

Journal of Molecular Endocrinology – Bioscientifica

Published: Oct 1, 1993

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