Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Effect of parathyroid hormone and calcitonin on the cytoplasmic spreading of isolated osteoclasts

Effect of parathyroid hormone and calcitonin on the cytoplasmic spreading of isolated osteoclasts ABSTRACTOsteoclasts, the major agents of bone resorption, were isolated from neonatal rat bone, and the cytoplasmic spreading of these cells was measured after incubation in the presence or absence of hormones or other cell types. Salmon calcitonin, which inhibits osteoclastic bone resorption, reduced spreading in a dose-dependent manner and caused significant inhibition at concentrations as low as 6·7 pg/ml. Parathyroid hormone (PTH) had no effect on the spreading of isolated osteoclasts but if osteoblasts and osteoclasts were co-cultured the addition of PTH caused a marked increase in spreading at concentrations of 0·025 i.u./ml and above. The results suggest that while calcitonin is a direct inhibitor of osteoclastic activity, PTH may stimulate osteoclasts through a primary action on osteoblasts.J. Endocr. (1984) 102, 281–286 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Endocrinology Bioscientifica

Effect of parathyroid hormone and calcitonin on the cytoplasmic spreading of isolated osteoclasts

Download PDF
6 pages

Loading next page...
 
/lp/bioscientifica/effect-of-parathyroid-hormone-and-calcitonin-on-the-cytoplasmic-A45dh39b6W

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Bioscientifica
Copyright
Copyright © 1984 The Authors. All Rights Reserved.
ISSN
0022-0795
eISSN
1479-6805
DOI
10.1677/joe.0.1020281
Publisher site
See Article on Publisher Site

Abstract

ABSTRACTOsteoclasts, the major agents of bone resorption, were isolated from neonatal rat bone, and the cytoplasmic spreading of these cells was measured after incubation in the presence or absence of hormones or other cell types. Salmon calcitonin, which inhibits osteoclastic bone resorption, reduced spreading in a dose-dependent manner and caused significant inhibition at concentrations as low as 6·7 pg/ml. Parathyroid hormone (PTH) had no effect on the spreading of isolated osteoclasts but if osteoblasts and osteoclasts were co-cultured the addition of PTH caused a marked increase in spreading at concentrations of 0·025 i.u./ml and above. The results suggest that while calcitonin is a direct inhibitor of osteoclastic activity, PTH may stimulate osteoclasts through a primary action on osteoblasts.J. Endocr. (1984) 102, 281–286

Journal

Journal of Endocrinology – Bioscientifica

Published: Sep 1, 1984

There are no references for this article.