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BREAD: A DIET FOR USE IN THE McKENZIE BIOASSAY

BREAD: A DIET FOR USE IN THE McKENZIE BIOASSAY Section of Pharmacology, Academic Division of Medicine and *Field Laboratories, The University, Sheffield, S102TN(Received 8 July 1975)The McKenzie (1958) bioassay remains the principal tool for studying the long-acting thyroid stimulator (LATS) and LATS-protector (LATSP), although radioreceptor binding assays are being developed (Manley, Bourke & Hawker, 1974; Smith & Hall, 1974). A dog biscuit met the low-iodine requirements until 1974, when batches contained sufficient iodine to make them unsuitable for use in this bioassay. Bread contains negligible iodine, unless iodated dough conditioner is added (London & Vought, 1965). Thus an investigation was made of the suitability of bread for use in the McKenzie bioassay.Female, white Swiss mice were bred as described by Loy & Broadhead (1968), the colony being fed on pasteurized breeding diet (Oxoid Ltd). Mice were weaned at 4 weeks, fed on diet 86 (Oxoid Ltd) until body weights were 15–20 g (i.e. usually for 1–2 weeks), http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Endocrinology Bioscientifica

BREAD: A DIET FOR USE IN THE McKENZIE BIOASSAY

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Publisher
Bioscientifica
Copyright
Copyright © 1975 The Authors. All Rights Reserved.
ISSN
0022-0795
eISSN
1479-6805
DOI
10.1677/joe.0.0670309
Publisher site
See Article on Publisher Site

Abstract

Section of Pharmacology, Academic Division of Medicine and *Field Laboratories, The University, Sheffield, S102TN(Received 8 July 1975)The McKenzie (1958) bioassay remains the principal tool for studying the long-acting thyroid stimulator (LATS) and LATS-protector (LATSP), although radioreceptor binding assays are being developed (Manley, Bourke & Hawker, 1974; Smith & Hall, 1974). A dog biscuit met the low-iodine requirements until 1974, when batches contained sufficient iodine to make them unsuitable for use in this bioassay. Bread contains negligible iodine, unless iodated dough conditioner is added (London & Vought, 1965). Thus an investigation was made of the suitability of bread for use in the McKenzie bioassay.Female, white Swiss mice were bred as described by Loy & Broadhead (1968), the colony being fed on pasteurized breeding diet (Oxoid Ltd). Mice were weaned at 4 weeks, fed on diet 86 (Oxoid Ltd) until body weights were 15–20 g (i.e. usually for 1–2 weeks),

Journal

Journal of Endocrinology – Bioscientifica

Published: Nov 1, 1975

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