The DNA Translocating Vertex of DSDNA Bacteriophage

The DNA Translocating Vertex of DSDNA Bacteriophage The transfer of DNA from one prokaryotic cell compartment to another takes place during mating, during phage assembly, and possibly during cell division. During the assembly of double-stranded DNA phages, DNA molecules are not 0066-4227 /85/1001-0109$02.00 BAZINET & KING only translocated from the cell into the phage capsid but are condensed into a tightly wound state (26). The discovery that the shells of bacteriophages were assembled empty of DNA, and that the DNA was subsequently threaded into the precursor shells, established an active role for precursor shells in the DNA packaging process (19, 76). At the heart of both processes-shell assembly and DNA packaging­ is the singular vertex that morphologically forms the site of tail attachment. In their 1978 review, Murialdo & Becker (76) focused attention on this vertex as a key to understanding both shell assembly and DNA packaging. The vertex functions in at least three different phases of the phage life-cycle: (a) initiation of procapsid assembly; (b) encapsidation of DNA into the pro­ capsid; and (c) transfer of the DNA out of the capsid into the host cell. We will refer to this vertex as the DNA translocating or portal vertex. In the mature phage the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Microbiology Annual Reviews

The DNA Translocating Vertex of DSDNA Bacteriophage

Annual Review of Microbiology, Volume 39 (1) – Oct 1, 1985

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Publisher
Annual Reviews
Copyright
Copyright 1985 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
0066-4227
eISSN
1545-3251
DOI
10.1146/annurev.mi.39.100185.000545
Publisher site
See Article on Publisher Site

Abstract

The transfer of DNA from one prokaryotic cell compartment to another takes place during mating, during phage assembly, and possibly during cell division. During the assembly of double-stranded DNA phages, DNA molecules are not 0066-4227 /85/1001-0109$02.00 BAZINET & KING only translocated from the cell into the phage capsid but are condensed into a tightly wound state (26). The discovery that the shells of bacteriophages were assembled empty of DNA, and that the DNA was subsequently threaded into the precursor shells, established an active role for precursor shells in the DNA packaging process (19, 76). At the heart of both processes-shell assembly and DNA packaging­ is the singular vertex that morphologically forms the site of tail attachment. In their 1978 review, Murialdo & Becker (76) focused attention on this vertex as a key to understanding both shell assembly and DNA packaging. The vertex functions in at least three different phases of the phage life-cycle: (a) initiation of procapsid assembly; (b) encapsidation of DNA into the pro­ capsid; and (c) transfer of the DNA out of the capsid into the host cell. We will refer to this vertex as the DNA translocating or portal vertex. In the mature phage the

Journal

Annual Review of MicrobiologyAnnual Reviews

Published: Oct 1, 1985

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