5 -10 L---'--:-:'--::-- '---::-ï¿½=--l...-.,,-J 2'-= --'----:: o=--.L---' 2 2 '8 2,.'-4 00 0 0 Wavelength (nm) /.//",\\ !,/ \ i! \ /: I .I ./'.\ ,. .... i .\ > Figure 1 The CD of myoglobin (dots and dashes), triosephosphate isomerase (dotted line), lysozyme (dashed line), and IX-chymotrypsin (solid line), redrawn from Brahms & Brahms (14) and Hennessey & Johnson (40). CD OF PROTEINS CIRCULAR DICHROISM SPECTROSCOPY Technique Normal absorption spectroscopy of electronic transitions is measured as the absorbance, A, which depends on the concentration of the material,c, and the pathlength of the cell,I. The constant of proportionality between the measurement and the variables is a characteristic of the molecule called the extinction coefficient, e, which varies with wavelength,A. The relationship can be summed up in Beer's Law, A(A) e(A)lc, where A has no unit, I is in em, c is in mol liter-l (per monomer for polymers), and thus e is in liter mol-I cm -I. A hypothetical normal absorption spectrum is given in Figure 2. CD of a molecule is defined as the difference between the extinction coefficients for left and right circularly polarized light. Each type of light obeys Beer's Law,so the difference is given
Annual Review of Biophysics – Annual Reviews
Published: Jun 1, 1988
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