Secondary Structure of Proteins Through Circular Dichroism Spectroscopy

Secondary Structure of Proteins Through Circular Dichroism Spectroscopy 5 -10 L---'--:-:'--::-- '---::-�=--l...-.,,-J 2'-= --'----:: o=--.L---' 2 2 '8 2,.'-4 00 0 0 Wavelength (nm) /.//",\\ !,/ \ i! \ /: I .I ./'.\ ,. .... i .\ > Figure 1 The CD of myoglobin (dots and dashes), triosephosphate isomerase (dotted line), lysozyme (dashed line), and IX-chymotrypsin (solid line), redrawn from Brahms & Brahms (14) and Hennessey & Johnson (40). CD OF PROTEINS CIRCULAR DICHROISM SPECTROSCOPY Technique Normal absorption spectroscopy of electronic transitions is measured as the absorbance, A, which depends on the concentration of the material,c, and the pathlength of the cell,I. The constant of proportionality between the measurement and the variables is a characteristic of the molecule called the extinction coefficient, e, which varies with wavelength,A. The relationship can be summed up in Beer's Law, A(A) e(A)lc, where A has no unit, I is in em, c is in mol liter-l (per monomer for polymers), and thus e is in liter mol-I cm -I. A hypothetical normal absorption spectrum is given in Figure 2. CD of a molecule is defined as the difference between the extinction coefficients for left and right circularly polarized light. Each type of light obeys Beer's Law,so the difference is given http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Biophysics Annual Reviews

Secondary Structure of Proteins Through Circular Dichroism Spectroscopy

Annual Review of Biophysics, Volume 17 (1) – Jun 1, 1988

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Publisher
Annual Reviews
Copyright
Copyright 1988 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
1936-122X
eISSN
1936-1238
DOI
10.1146/annurev.bb.17.060188.001045
Publisher site
See Article on Publisher Site

Abstract

5 -10 L---'--:-:'--::-- '---::-�=--l...-.,,-J 2'-= --'----:: o=--.L---' 2 2 '8 2,.'-4 00 0 0 Wavelength (nm) /.//",\\ !,/ \ i! \ /: I .I ./'.\ ,. .... i .\ > Figure 1 The CD of myoglobin (dots and dashes), triosephosphate isomerase (dotted line), lysozyme (dashed line), and IX-chymotrypsin (solid line), redrawn from Brahms & Brahms (14) and Hennessey & Johnson (40). CD OF PROTEINS CIRCULAR DICHROISM SPECTROSCOPY Technique Normal absorption spectroscopy of electronic transitions is measured as the absorbance, A, which depends on the concentration of the material,c, and the pathlength of the cell,I. The constant of proportionality between the measurement and the variables is a characteristic of the molecule called the extinction coefficient, e, which varies with wavelength,A. The relationship can be summed up in Beer's Law, A(A) e(A)lc, where A has no unit, I is in em, c is in mol liter-l (per monomer for polymers), and thus e is in liter mol-I cm -I. A hypothetical normal absorption spectrum is given in Figure 2. CD of a molecule is defined as the difference between the extinction coefficients for left and right circularly polarized light. Each type of light obeys Beer's Law,so the difference is given

Journal

Annual Review of BiophysicsAnnual Reviews

Published: Jun 1, 1988

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