DNA Packaging in dsDNA Bacteriophages

DNA Packaging in dsDNA Bacteriophages Packaging of duplex DNA within the icosahedral phage head has long been of interest as the prokaryotic equivalent of chromosome condensation. More recently, it has received attention because of its usefulness in vitro in recom­ binant DNA work. This DNA condensation results from a complex and highly evolved biological process whose mechanisms depart in numerous ways from 0066-4227/89/100 1 -0267$02.00 BLACK simple self-assembly. dsDNA phages displaying broadly similar overall packaging mechanisms and packaged DNA structure include }o.., T4, P22, T7 and T3 , c!>29, P2, T l , Mu, PI, and others that have been less intensively studied. Recent work has emphasized five related but distinct areas: (a) the mech­ anism and energetics of DNA translocation into the prohead precursor; (b) the higher order structure(s) of the condensed DNA within the capsid; (c) the mechanism of DNA end formation by concatemer cutting at cohesive end sites (cos) or packaging sites (pac) and by headful cutting, and control in vivo of concatemer cutting and packaging; (d) the relationship of DNA packaging to global DNA metabolism and structure in infected bacteria; and (e) use of in vitro packaging systems in recombinant DNA constructions and cloning work. Accordingly, these interests are http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Microbiology Annual Reviews

DNA Packaging in dsDNA Bacteriophages

Annual Review of Microbiology, Volume 43 (1) – Oct 1, 1989

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Publisher
Annual Reviews
Copyright
Copyright 1989 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
0066-4227
eISSN
1545-3251
DOI
10.1146/annurev.mi.43.100189.001411
Publisher site
See Article on Publisher Site

Abstract

Packaging of duplex DNA within the icosahedral phage head has long been of interest as the prokaryotic equivalent of chromosome condensation. More recently, it has received attention because of its usefulness in vitro in recom­ binant DNA work. This DNA condensation results from a complex and highly evolved biological process whose mechanisms depart in numerous ways from 0066-4227/89/100 1 -0267$02.00 BLACK simple self-assembly. dsDNA phages displaying broadly similar overall packaging mechanisms and packaged DNA structure include }o.., T4, P22, T7 and T3 , c!>29, P2, T l , Mu, PI, and others that have been less intensively studied. Recent work has emphasized five related but distinct areas: (a) the mech­ anism and energetics of DNA translocation into the prohead precursor; (b) the higher order structure(s) of the condensed DNA within the capsid; (c) the mechanism of DNA end formation by concatemer cutting at cohesive end sites (cos) or packaging sites (pac) and by headful cutting, and control in vivo of concatemer cutting and packaging; (d) the relationship of DNA packaging to global DNA metabolism and structure in infected bacteria; and (e) use of in vitro packaging systems in recombinant DNA constructions and cloning work. Accordingly, these interests are

Journal

Annual Review of MicrobiologyAnnual Reviews

Published: Oct 1, 1989

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