Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-state absorption, stimulated emission, and ground-state depletion), provides new image contrasts for nonfluorescent species. Thanks to the high-frequency modulation transfer scheme, these imaging techniques exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Physical Chemistry Annual Reviews

Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

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Publisher
Annual Reviews
Copyright
Copyright ©© 2011 by Annual Reviews. All rights reserved
ISSN
0066-426X
eISSN
1545-1593
D.O.I.
10.1146/annurev.physchem.012809.103512
Publisher site
See Article on Publisher Site

Abstract

The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-state absorption, stimulated emission, and ground-state depletion), provides new image contrasts for nonfluorescent species. Thanks to the high-frequency modulation transfer scheme, these imaging techniques exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques.

Journal

Annual Review of Physical ChemistryAnnual Reviews

Published: May 5, 2011

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