In 1964 Sachs and Takabatake (18, 19) suggested that vasopressin (VP) and its associated neurophysin "carrier protein" were produced as parts of a common precursor molecule. Subsequently, it was proposed that oxytocin and its neurophysin were made by a similar mechanism precursors escaped detection until the (12). The putative 19708, however, when they were identified in the course of pulse-chase studies (2-5). The nucleotide seÂ oxytocinlneurophysin precursor (prooxyphysin) remains to be elucidated. quence of cloned cDNA encoding the bovine vasopressin/neurophysin preÂ cursor (propressophysin) has been determined (7); the structure of the CHARACTERIZATION OF NEUROHYPOPHYSIAL PROHORMONES Prior to the cloning and sequencing of propressophysin eDNA, a good deal of information had already been obtained about the structure of the proÂ pressophysin molecule. Three general strategies were employed for studying this protein. Lauber, Cohen, and their colleagues (8, 10) made bovine neurohypophyseal extracts, separated the proteins in these extracts chromatographically, and assayed for neurophysin- and vasopressin-like molecules by radioimmunoassay. Russell, Gainer, and Brownstein (2-5, 13-16) employed pulse-chase methodology. They infused 35S-cysteine into the brains of rats adjacent to their supraoptic nuclei, and looked for newly formed cysteine-rich molecules that behaved like neurophysin precursors. Finally, Schmale & Richter (20-24) extracted mRNA
Annual Review of Physiology – Annual Reviews
Published: Mar 1, 1983
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