Biosynthesis of Vasopressin and Oxytocin

Biosynthesis of Vasopressin and Oxytocin In 1964 Sachs and Takabatake (18, 19) suggested that vasopressin (VP) and its associated neurophysin "carrier protein" were produced as parts of a common precursor molecule. Subsequently, it was proposed that oxytocin and its neurophysin were made by a similar mechanism precursors escaped detection until the (12). The putative 19708, however, when they were identified in the course of pulse-chase studies (2-5). The nucleotide se­ oxytocinlneurophysin precursor (prooxyphysin) remains to be elucidated. quence of cloned cDNA encoding the bovine vasopressin/neurophysin pre­ cursor (propressophysin) has been determined (7); the structure of the CHARACTERIZATION OF NEUROHYPOPHYSIAL PROHORMONES Prior to the cloning and sequencing of propressophysin eDNA, a good deal of information had already been obtained about the structure of the pro­ pressophysin molecule. Three general strategies were employed for studying this protein. Lauber, Cohen, and their colleagues (8, 10) made bovine neurohypophyseal extracts, separated the proteins in these extracts chromatographically, and assayed for neurophysin- and vasopressin-like molecules by radioimmunoassay. Russell, Gainer, and Brownstein (2-5, 13-16) employed pulse-chase methodology. They infused 35S-cysteine into the brains of rats adjacent to their supraoptic nuclei, and looked for newly formed cysteine-rich molecules that behaved like neurophysin precursors. Finally, Schmale & Richter (20-24) extracted mRNA http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Physiology Annual Reviews

Biosynthesis of Vasopressin and Oxytocin

Annual Review of Physiology, Volume 45 (1) – Mar 1, 1983

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Publisher
Annual Reviews
Copyright
Copyright 1983 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
0066-4278
eISSN
1545-1585
DOI
10.1146/annurev.ph.45.030183.001021
pmid
6847162
Publisher site
See Article on Publisher Site

Abstract

In 1964 Sachs and Takabatake (18, 19) suggested that vasopressin (VP) and its associated neurophysin "carrier protein" were produced as parts of a common precursor molecule. Subsequently, it was proposed that oxytocin and its neurophysin were made by a similar mechanism precursors escaped detection until the (12). The putative 19708, however, when they were identified in the course of pulse-chase studies (2-5). The nucleotide se­ oxytocinlneurophysin precursor (prooxyphysin) remains to be elucidated. quence of cloned cDNA encoding the bovine vasopressin/neurophysin pre­ cursor (propressophysin) has been determined (7); the structure of the CHARACTERIZATION OF NEUROHYPOPHYSIAL PROHORMONES Prior to the cloning and sequencing of propressophysin eDNA, a good deal of information had already been obtained about the structure of the pro­ pressophysin molecule. Three general strategies were employed for studying this protein. Lauber, Cohen, and their colleagues (8, 10) made bovine neurohypophyseal extracts, separated the proteins in these extracts chromatographically, and assayed for neurophysin- and vasopressin-like molecules by radioimmunoassay. Russell, Gainer, and Brownstein (2-5, 13-16) employed pulse-chase methodology. They infused 35S-cysteine into the brains of rats adjacent to their supraoptic nuclei, and looked for newly formed cysteine-rich molecules that behaved like neurophysin precursors. Finally, Schmale & Richter (20-24) extracted mRNA

Journal

Annual Review of PhysiologyAnnual Reviews

Published: Mar 1, 1983

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