Neil G. Grant 2 and Max H. Hommersand a Department of Botany, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514 Abstract The respiration and cytochrome properties of “glucose-bleached” Chlorella protothecoides Krüger, Indiana strain 25, were studied. This organism, when grown heterotrophically with high glucose and a low organic nitrogen source, has no chlorophyll, little carotenoid, and diminished chloroplast structure—factors which make it suitable for respiration studies. Whole cell endogenous oxygen uptake rates are either stimulated or only slightly inhibited by cyanide, azide, CO, and antimycin. When these inhibitors are used with m -chlorobenz-hydroxamic acid ( m CLAM), an inhibitor of higher plant mitochondrial alternate oxidase, O 2 uptake is inhibited. There is little effect of m CLAM by itself on the rate of oxygen uptake. The inhibition by CO of O 2 uptake in the presence of m CLAM is reversed by light. The cytochrome chain of C. protothecoides consists of cytochromes aa 3 , b , and c , as revealed by room temperature difference spectra. In common with mitochondria of higher plants, there is a further reduction of cytochrome b with dithionite. In the presence of antimycin, the cytochromes aa 3 and c are oxidized and cytochrome b is reduced. Cyanide causes a partial reduction of cytochromes aa 3 and c while cytochrome b remains oxidized. This general response is characteristic of higher plant mitochrondria having large amounts of cyanide-resistant respiration. Carbon monoxide spectra reveal one CO-combining pigment. The cytochrome b region differs from that of higher plants in that the typical complex spectrum does not appear at low temperature (−190 C). The concentration of cytochrome aa 3 per cell volume was observed during the greening of “glucose-bleached” cells. The concentration of these cytochromes nearly tripled during the 24 hours of the initial stages of greening.
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera