Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice

Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice We identified a gene responsible for tolerance to boron (B) toxicity in rice ( Oryza sativa ), named BORON EXCESS TOLERANT1 . Using recombinant inbred lines derived from the B-toxicity-sensitive indica -ecotype cultivar IR36 and the tolerant japonica -ecotype cultivar Nekken 1, the region responsible for tolerance to B toxicity was narrowed to 49 kb on chromosome 4. Eight genes are annotated in this region. The DNA sequence in this region was compared between the B-toxicity-sensitive japonica cultivar Wataribune and the B-toxicity-tolerant japonica cultivar Nipponbare by eco-TILLING analysis and revealed a one-base insertion mutation in the open reading frame sequence of the gene Os04g0477300. The gene encodes a NAC (NAM, ATAF, and CUC)-like transcription factor and the function of the transcript is abolished in B-toxicity-tolerant cultivars. Transgenic plants in which the expression of Os04g0477300 is abolished by RNA interference gain tolerance to B toxicity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Suppression of a NAC-Like Transcription Factor Gene Improves Boron-Toxicity Tolerance in Rice

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
D.O.I.
10.1104/pp.110.171470
Publisher site
See Article on Publisher Site

Abstract

We identified a gene responsible for tolerance to boron (B) toxicity in rice ( Oryza sativa ), named BORON EXCESS TOLERANT1 . Using recombinant inbred lines derived from the B-toxicity-sensitive indica -ecotype cultivar IR36 and the tolerant japonica -ecotype cultivar Nekken 1, the region responsible for tolerance to B toxicity was narrowed to 49 kb on chromosome 4. Eight genes are annotated in this region. The DNA sequence in this region was compared between the B-toxicity-sensitive japonica cultivar Wataribune and the B-toxicity-tolerant japonica cultivar Nipponbare by eco-TILLING analysis and revealed a one-base insertion mutation in the open reading frame sequence of the gene Os04g0477300. The gene encodes a NAC (NAM, ATAF, and CUC)-like transcription factor and the function of the transcript is abolished in B-toxicity-tolerant cultivars. Transgenic plants in which the expression of Os04g0477300 is abolished by RNA interference gain tolerance to B toxicity.

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