Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium.

Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium. Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ((Ca2+)i) are transduced into different physiological responses remains to be explained. Oscillations in (Ca2+)i may provide one way in which this can occur. We used photometric and imaging techniques to examine this hypothesis in guard cells of Commelina communis. External Ca2+ ((Ca2+)e), which causes an increase in (Ca2+)i, was used as a closing stimulus. The total increase in (Ca2+)i was directly related to the concentration of (Ca2+)e, both of which correlated closely with the degree of stomatal closure. Increases were oscillatory in nature, with the pattern of the oscillations dependent on the concentration of (Ca2+)e. At 0.1 mM, (Ca2+)e induced symmetrical oscillations. In contrast, 1.0 mM (Ca2+)e induced asymmetric oscillations. Oscillations were stimulus dependent and modulated by changing (Ca2+)e. Experiments using Ca2+ channel blockers and Mn2+-quenching studies suggested a role for Ca2+ influx during the oscillatory behavior without excluding the possible involvement of Ca2+ release from intracellular stores. These data suggest a mechanism for encoding the information required to distinguish between a number of different Ca2+-mobilizing stimuli in guard cells, using stimulus-specific patterns of oscillations in (Ca2+)i. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Stimulus-Induced Oscillations in Guard Cell Cytosolic Free Calcium.

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1995 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.7.8.1207
Publisher site
See Article on Publisher Site

Abstract

Ca2+ is implicated as a second messenger in the response of stomata to a range of stimuli. However, the mechanism by which stimulus-induced increases in guard cell cytosolic free Ca2+ ((Ca2+)i) are transduced into different physiological responses remains to be explained. Oscillations in (Ca2+)i may provide one way in which this can occur. We used photometric and imaging techniques to examine this hypothesis in guard cells of Commelina communis. External Ca2+ ((Ca2+)e), which causes an increase in (Ca2+)i, was used as a closing stimulus. The total increase in (Ca2+)i was directly related to the concentration of (Ca2+)e, both of which correlated closely with the degree of stomatal closure. Increases were oscillatory in nature, with the pattern of the oscillations dependent on the concentration of (Ca2+)e. At 0.1 mM, (Ca2+)e induced symmetrical oscillations. In contrast, 1.0 mM (Ca2+)e induced asymmetric oscillations. Oscillations were stimulus dependent and modulated by changing (Ca2+)e. Experiments using Ca2+ channel blockers and Mn2+-quenching studies suggested a role for Ca2+ influx during the oscillatory behavior without excluding the possible involvement of Ca2+ release from intracellular stores. These data suggest a mechanism for encoding the information required to distinguish between a number of different Ca2+-mobilizing stimuli in guard cells, using stimulus-specific patterns of oscillations in (Ca2+)i.

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