Respiratory Chain of Plant Mitochondria XVIII. Point of Interaction of the Alternate Oxidase with the Respiratory Chain

Respiratory Chain of Plant Mitochondria XVIII. Point of Interaction of the Alternate Oxidase with... Bayard T. Storey 2 a Departments of Obstetrics and Gynecology, Physiology, and Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174 Abstract Oxidation of the respiratory chain carriers of anaerobic, CO-saturated skunk cabbage ( Symplocarpus foetidus ) mitochondria, by means of an O 2 pulse, proceeds primarily through the cyanide-insensitive alternate oxidase, since the oxidation of cytochromes a and a 3 takes place with a half-time of 3 seconds, corresponding to the rate of dissociation of CO from reduced cytochrome a 3 . Ubiquinone and part of the flavoprotein are oxidized within 1 second under these conditions, and this rapid rate of oxidation is strongly inhibited by m -chlorobenzhydroxamic acid ( m CLAM), a specific inhibitor of the alternate oxidase of plant mitochondria. The rate of ubiquinone oxidation under these conditions in white potato ( Solanum tuberosum ) mitochondria, which have no alternate oxidase, is the same as that in skunk cabbage mitochondria treated with m CLAM. Ubiquinone, thus identified as the carrier common to both the cytochrome and alternate oxidase pathways, is linked to the alternate oxidase by a flavoprotein of midpoint potential 50 millivolts more negative with which it is in equilibrium. This arrangement provides a switch for diverting electron transport primarily through the cytochrome pathway under state 3 conditions and primarily through the alternate oxidase pathway under state 4 conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Respiratory Chain of Plant Mitochondria XVIII. Point of Interaction of the Alternate Oxidase with the Respiratory Chain

Oct 1, 1976

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Publisher
American Society of Plant Biologist
Copyright
Copyright © 1976 by the American Society of Plant Biologists
ISSN
1532-2548
eISSN
0032-0889
DOI
10.1104/pp.58.4.521
Publisher site
See Article on Publisher Site

Abstract

Bayard T. Storey 2 a Departments of Obstetrics and Gynecology, Physiology, and Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19174 Abstract Oxidation of the respiratory chain carriers of anaerobic, CO-saturated skunk cabbage ( Symplocarpus foetidus ) mitochondria, by means of an O 2 pulse, proceeds primarily through the cyanide-insensitive alternate oxidase, since the oxidation of cytochromes a and a 3 takes place with a half-time of 3 seconds, corresponding to the rate of dissociation of CO from reduced cytochrome a 3 . Ubiquinone and part of the flavoprotein are oxidized within 1 second under these conditions, and this rapid rate of oxidation is strongly inhibited by m -chlorobenzhydroxamic acid ( m CLAM), a specific inhibitor of the alternate oxidase of plant mitochondria. The rate of ubiquinone oxidation under these conditions in white potato ( Solanum tuberosum ) mitochondria, which have no alternate oxidase, is the same as that in skunk cabbage mitochondria treated with m CLAM. Ubiquinone, thus identified as the carrier common to both the cytochrome and alternate oxidase pathways, is linked to the alternate oxidase by a flavoprotein of midpoint potential 50 millivolts more negative with which it is in equilibrium. This arrangement provides a switch for diverting electron transport primarily through the cytochrome pathway under state 3 conditions and primarily through the alternate oxidase pathway under state 4 conditions.

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