Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper

Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH... Plants recruit innate immune receptors such as leucine-rich repeat ( LRR ) proteins to recognize pathogen attack and activate defense genes. Here, we identified the pepper ( Capsicum annuum ) pathogenesis-related protein10 (PR10) as a leucine-rich repeat protein1 (LRR1)–interacting partner. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the specific interaction between LRR1 and PR10 in planta. Avirulent Xanthomonas campestris pv vesicatoria infection induces PR10 expression associated with the hypersensitive cell death response. Transient expression of PR10 triggers hypersensitive cell death in pepper and Nicotiana benthamiana leaves, which is amplified by LRR1 coexpression as a positive regulator. LRR1 promotes the ribonuclease activity and phosphorylation of PR10, leading to enhanced cell death signaling. The LRR1-PR10 complex is formed in the cytoplasm, resulting in its secretion into the apoplastic space. Engineered nuclear confinement of both proteins revealed that the cytoplasmic localization of the PR10-LRR1 complex is essential for cell death–mediated defense signaling. PR10 / LRR1 silencing in pepper compromises resistance to avirulent X. campestris pv vesicatoria infection. By contrast, PR10 / LRR1 overexpression in Arabidopsis thaliana confers enhanced resistance to Pseudomonas syringae pv tomato and Hyaloperonospora arabidopsidis . Together, these results suggest that the cytosolic LRR-PR10 complex is responsible for cell death–mediated defense signaling. Glossary PCD programmed cell death HR hypersensitive response LRR leucine-rich repeat NB nucleotide binding domain RLK receptor-like kinase Xcv Xanthomonas campestris pv vesicatoria ROS reactive oxygen species SA salicylic acid NLS nuclear localization signal BiFC bimolecular fluorescence complementation VIGS virus-induced gene silencing YFP yellow fluorescent protein co-IP coimmunoprecipitation GFP green fluorescent protein GST glutathione S -transferase BFA brefeldin A DAB 3,3′-diaminobenzidine tetrahydrochloride H 2 O 2 hydrogen peroxide Col-0 Columbia-0 OX overexpression Pst P. syringae pv tomato AD activation domain BD binding domain DAPI 4′,6-diamidino-2-phenylindole TRV tobacco rattle virus cfu colony-forming units http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper

Loading next page...
 
/lp/american-society-of-plant-biologist/requirement-of-the-cytosolic-interaction-between-pathogenesis-related-a5oux5h4lt
Publisher
American Society of Plant Biologist
Copyright
Copyright © 2015 by the American Society of Plant Biologists
ISSN
1040-4651
eISSN
1532-298X
D.O.I.
10.1105/tpc.112.095869
Publisher site
See Article on Publisher Site

Abstract

Plants recruit innate immune receptors such as leucine-rich repeat ( LRR ) proteins to recognize pathogen attack and activate defense genes. Here, we identified the pepper ( Capsicum annuum ) pathogenesis-related protein10 (PR10) as a leucine-rich repeat protein1 (LRR1)–interacting partner. Bimolecular fluorescence complementation and coimmunoprecipitation assays confirmed the specific interaction between LRR1 and PR10 in planta. Avirulent Xanthomonas campestris pv vesicatoria infection induces PR10 expression associated with the hypersensitive cell death response. Transient expression of PR10 triggers hypersensitive cell death in pepper and Nicotiana benthamiana leaves, which is amplified by LRR1 coexpression as a positive regulator. LRR1 promotes the ribonuclease activity and phosphorylation of PR10, leading to enhanced cell death signaling. The LRR1-PR10 complex is formed in the cytoplasm, resulting in its secretion into the apoplastic space. Engineered nuclear confinement of both proteins revealed that the cytoplasmic localization of the PR10-LRR1 complex is essential for cell death–mediated defense signaling. PR10 / LRR1 silencing in pepper compromises resistance to avirulent X. campestris pv vesicatoria infection. By contrast, PR10 / LRR1 overexpression in Arabidopsis thaliana confers enhanced resistance to Pseudomonas syringae pv tomato and Hyaloperonospora arabidopsidis . Together, these results suggest that the cytosolic LRR-PR10 complex is responsible for cell death–mediated defense signaling. Glossary PCD programmed cell death HR hypersensitive response LRR leucine-rich repeat NB nucleotide binding domain RLK receptor-like kinase Xcv Xanthomonas campestris pv vesicatoria ROS reactive oxygen species SA salicylic acid NLS nuclear localization signal BiFC bimolecular fluorescence complementation VIGS virus-induced gene silencing YFP yellow fluorescent protein co-IP coimmunoprecipitation GFP green fluorescent protein GST glutathione S -transferase BFA brefeldin A DAB 3,3′-diaminobenzidine tetrahydrochloride H 2 O 2 hydrogen peroxide Col-0 Columbia-0 OX overexpression Pst P. syringae pv tomato AD activation domain BD binding domain DAPI 4′,6-diamidino-2-phenylindole TRV tobacco rattle virus cfu colony-forming units

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off